Immunocytochemical detection of oncomodulin in tumor tissue.

Abstract

Using the rat hepatoma calcium-binding protein, oncomodulin, from Morris hepatoma 5123tc, an antiserum has been raised in rabbits useful for immunostaining of this tumor protein. The peroxidase-antiperoxidase (PAP) technique has been used to demonstrate oncomodulin in sections of Morris rat hepatomas 5123D, 5123tc, 7288Ctc, and 7777 fixed with Bouin's or Carnoy's fixatives, or using freeze substitution. Oncomodulin-specific staining was also shown by a hepatoma metastasis in lung. Optimal conditions for the indirect fluorescent antibody technique were established to demonstrate oncomodulin in virally transformed NRK cells (ASV-NRK). In both tumors and cultured neoplastic cells staining appeared which suggested that oncomodulin might occur in nucleus and cytoplasm. Normal untransformed tissues and uninfected cells did not show oncomodulin staining.

• Authors: L M Brewer, J P Durkin, J P MacManus
• Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (J Histochem Cytochem) Vol. 32 Issue 10 Pg. 1009-16 (Oct 1984) ISSN: 0022-1554 [Print] United States
• PMID: 6384359 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Calcium-Binding Proteins
• Neoplasm Proteins
• oncomodulin

Topics

• Animals
• Calcium-Binding Proteins (analysis)
• Cell Line
• Fluorescent Antibody Technique
• Immunoenzyme Techniques
• Liver Neoplasms, Experimental (analysis, pathology)
• Neoplasm Proteins (analysis)
• Radioimmunoassay
• Rats
• Rats, Inbred BUF