[Change in the molecular properties of sarcoplasmic reticulum Ca-ATPase during modification of the membranes with cholesterol].

Abstract

The thiol reagent NBD-chloride (4-chloro-7-nitro-benzo-2-oxo-1,3-diazole) was used to determine the amount and reactivity of SH-groups of Ca-ATPase of rat skeletal muscle sarcoplasmic reticulum during hypercholesterolemia. Modification of membranes with cholesterol brought about a decrease in the total amount and reactivity of SH-groups at the cost of reduction of rapid SH-groups and decrease of the modification constant of these SH-groups. The masking effect of high concentrations of ATP on the reactivity of SH-groups in hypercholesterolemia was noticed. It is inferred that the reduced efficacy of Ca-pump work found under the same experimental conditions before is a consequence of the modification of sarcoplasmic reticulum membranes with cholesterol and change in the molecular conformation of Ca-ATPase.

• Authors: L V Stoĭda
• Journal: Biulleten' eksperimental'noi biologii i meditsiny (Biull Eksp Biol Med) Vol. 96 Issue 10 Pg. 38-40 (Oct 1983) ISSN: 0365-9615 [Print] Russia (Federation)
• Vernacular Title: Izmenenie molekuliarnykh svoĭstv Ca-ATFazy sarkoplazmaticheskogo retikuluma pri modifikatsii membran kholesterinom.
• PMID: 6226328 (Publication Type: Journal Article)

Chemical References

• Cholesterol, Dietary
• Sulfhydryl Compounds
• Calcium-Transporting ATPases

Topics

• Animals
• Calcium-Transporting ATPases (analysis)
• Cholesterol, Dietary (administration & dosage)
• Hypercholesterolemia (enzymology)
• Male
• Molecular Conformation
• Rabbits
• Sarcoplasmic Reticulum (enzymology)
• Sulfhydryl Compounds (analysis)