Reproducible chromosome changes of polycyclic hydrocarbon-induced rat leukemia: incidence and chromosome banding pattern.

Abstract

Statistical analysis of 361 cases of primary leukemia induced in outbred Long-Evans and Sprague-Dawley rats by 7,12-dimethylbenz[a]anthracene (DMBA) and 7,8,12-trimethylbenz[a]anthracene (TMBA) showed that the incidence of trisomy of chromosome No. 2 was significantly lower with TMBA (17.8%) than with DMBA (29.3%). This tendency was reproducible in both sexes. Another characteristic chromosome abnormality, long No. 2, was found in 10 cases (2.8%). Quinacrine fluorescence analysis revealed that cells with No. 2 trisomy or either of two types of long No. 2 had total and partial No. 2 trisomy, respectively. Other chromosome members of cells with long No. 2, as well as the chromosomes of cells with typical No. 2 trisomy and "normal diploid" leukemia cells, revealed no band abnormality. The phenotype of No. 2 trisomy, severe anemia of the hosts reported in DMBA-induced leukemias, was also noted in leukemias with TMBA-induced No. 2 trisomy but not in leukemias with long No. 2.

• Authors: T Sugiyama, H Uenaka, N Ueda, S Fukuhara, S Maeda
• Journal: Journal of the National Cancer Institute (J Natl Cancer Inst) Vol. 60 Issue 1 Pg. 153-60 (Jan 1978) ISSN: 0027-8874 [Print] United States
• PMID: 415146 (Publication Type: Journal Article)

Chemical References

• Benz(a)Anthracenes
• 9,10-Dimethyl-1,2-benzanthracene
• Quinacrine

Topics

• 9,10-Dimethyl-1,2-benzanthracene (analogs & derivatives)
• Anemia (complications, genetics)
• Animals
• Benz(a)Anthracenes (analogs & derivatives)
• Chromosome Aberrations
• Female
• Fluorescence
• Leukemia, Experimental (chemically induced, complications, genetics)
• Male
• Quinacrine
• Rats
• Trisomy