Alpha 1-antitrypsin and serum albumin mRNA accumulation in normal, acute phase and ZZ human liver.

Abstract

Alpha 1-Antitrypsin and albumin mRNA levels of 4 human livers were assessed using a newly sequenced cDNA clone of the carboxyterminal third of alpha 1-antitrypsin and a previously cloned albumin cDNA sequence. The relative concentration of alpha 1-antitrypsin mRNA was the same in poly(A)-containing RNA isolated from acute phase (MM) and alpha1-antitrypsin deficient (ZZ) individuals. In the acute phase liver relative to the normal (MM) liver, total RNA extracts showed a marked decrease in albumin mRNA concentration but no increase in alpha 1-antitrypsin mRNA. The ZZ liver showed decreased total and poly(A)-containing RNA content but the same proportion of alpha 1-antitrypsin to albumin mRNA as in the normal (MM) liver. This supports other evidence that ZZ alpha 1-antitrypsin deficiency is due to a defect in polypeptide processing (secretion) rather than a deficiency in mRNA accumulation.

• Authors: J H Riley, I C Bathurst, M R Edbrooke, R W Carrell, R K Craig
• Journal: FEBS letters (FEBS Lett) Vol. 189 Issue 2 Pg. 361-6 (Sep 23 1985) ISSN: 0014-5793 [Print] England
• PMID: 3876243 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Messenger
• Serum Albumin
• alpha 1-Antitrypsin
• C-Reactive Protein
• DNA

Topics

• Adolescent
• Adult
• Amino Acid Sequence
• Base Sequence
• C-Reactive Protein (analysis)
• DNA (metabolism)
• Female
• Humans
• Liver (metabolism)
• Male
• Middle Aged
• RNA, Messenger (metabolism)
• Serum Albumin (genetics)
• Time Factors
• alpha 1-Antitrypsin (genetics)