Correlation of meniscus tear type with synovial inflammation and the therapeutic potential of docosapentaenoic acid.

Abstract

BACKGROUND:

Synovitis, characterized by inflammation of the synovial membrane, is commonly induced by meniscus tears. However, significant differences in inflammatory responses and the key inflammatory mediators of synovium induced by different types of meniscal tears remain unclear.

METHODS:

Magnetic resonance imaging (MRI) was employed to identify the type of meniscus tear, and the quantification of synovial inflammation was assessed through H&E staining assay. Transcription and expression levels of IL-1β and IL-6 were evaluated using bioinformatics, ELISA, RT-qPCR, and IHC of CD68 staining assays. The therapeutic potential of Docosapentaenoic Acid (DPA) was determined through network pharmacology, ELISA, and RT-qPCR assays. The safety of DPA was assessed using colony formation and EdU staining assays.

RESULTS:

The results indicate that both IL-1β and IL-6 play pivotal roles in synovitis pathogenesis, with distinct expression levels across various subtypes. Among tested meniscus tears, oblique tear and bucket handle tear induced the most severe inflammation, followed by radial tear and longitudinal tear, while horizontal tear resulted in the least inflammation. Furthermore, in synovial inflammation induced by specific meniscus tears, the anterior medial tissues exhibited significantly higher local inflammation than the anterior lateral and suprapatellar regions, highlighting the clinical relevance and practical guidance of anterior medial tissues' inflammatory levels. Additionally, we identified the essential omega-3 fatty acid DPA as a potential therapeutic agent for synovitis, demonstrating efficacy in blocking the transcription and expression of IL-1β and IL-6 with minimal side effects.

CONCLUSION:

These findings provide valuable insights into the nuanced nature of synovial inflammation induced by various meniscal tear classifications and contribute to the development of new adjunctive therapeutic agents in the management of synovitis.

• Authors: Lichuang Wu, Ming Ying, Yiheng Ye, Dongdong Wang, Chengwei Chen, Cailong Liu
• Journal: BMC musculoskeletal disorders (BMC Musculoskelet Disord) Vol. 25 Issue 1 Pg. 375 (May 11 2024) ISSN: 1471-2474 [Electronic] England
• PMID: 38734632 (Publication Type: Journal Article)
• Copyright: © 2024. The Author(s).

Chemical References

• docosapentaenoic acid
• Fatty Acids, Unsaturated
• Interleukin-1beta
• Interleukin-6

Topics

• Tibial Meniscus Injuries (drug therapy, metabolism)
• Synovitis (drug therapy, metabolism, pathology)
• Magnetic Resonance Imaging
• Synovial Membrane (drug effects, metabolism, pathology)
• Humans
• Fatty Acids, Unsaturated (pharmacology, metabolism, therapeutic use)
• Male
• Interleukin-1beta (metabolism)
• Animals
• Interleukin-6 (metabolism)
• Female
• Menisci, Tibial (drug effects, metabolism)
• Mice
• Disease Models, Animal