No-ozone cold plasma induces apoptosis in human neuroblastoma cell line via increased intracellular reactive oxygen species (ROS).

Abstract

BACKGROUND:

This study aimed to evaluate the effect of argon-based No-ozone Cold Plasma (NCP) on neuroblastoma cancer cell apoptosis.

METHODS:

Experiments were performed with SK-N-SH and HS 68. Cell cultures were treated with NCP for 1, 3, and 5 min. NCP was applied using three different strategies: direct NCP application to cell cultures, to only media, and to only cells. Evaluation of cell viability and the level of the reactive oxygen species (ROS) was performed. N-acetyl-L-cysteine (NAC) was also used to antagonize intracellular ROS. Cleaved caspase 3, PARP, aquaporin (AQP) 3 and 8 were detected.

RESULTS:

NCP induced a gradual decrease in the SK-N-SH cell viability. In contrast, the viability of HS 68 cells did not change. SK-N-SH cells viability was reduced the most when the only media-NCP application strategy was employed. Intracellular ROS levels were significantly increased with time. Cleaved caspase 3 and PARP were increased at 6 h after NCP application. SK-N-SH cells remained viable with NAC after NCP application. AQP 3 and 8 were over-expressed in SK-N-SH cells.

CONCLUSION:

These findings demonstrate the anti-cancer effect of NCP on neuroblastoma cells. NCP enhanced the selective apoptosis of neuroblastoma cells due to the increased intracellular ROS.

• Authors: Jung-Han Lee, M Shriya Jaiswal, Yoon-Seo Jang, Jeong-Hae Choi, Gyoo-Cheon Kim, Jin-Woo Hong, Dae-Seok Hwang
• Journal: BMC complementary medicine and therapies (BMC Complement Med Ther) Vol. 24 Issue 1 Pg. 46 (Jan 20 2024) ISSN: 2662-7671 [Electronic] England
• PMID: 38245726 (Publication Type: Journal Article)
• Copyright: © 2024. The Author(s).

Chemical References

• Reactive Oxygen Species
• Caspase 3
• Plasma Gases
• Ozone
• Poly(ADP-ribose) Polymerase Inhibitors
• Acetylcysteine

Topics

• Humans
• Reactive Oxygen Species (metabolism)
• Caspase 3 (metabolism)
• Plasma Gases (pharmacology, therapeutic use)
• Ozone (pharmacology, therapeutic use)
• Poly(ADP-ribose) Polymerase Inhibitors (pharmacology, therapeutic use)
• Cell Line, Tumor
• Apoptosis
• Neuroblastoma (drug therapy, metabolism)
• Acetylcysteine (pharmacology, therapeutic use)