Abstract | Background and aims: We aimed to evaluate the efficiency of identification and quantification of hemoglobin ( Hb) Constant Spring (CS) and Hb Paksé by capillary electrophoresis (CE). Materials and methods: Blood samples collected from 2057 patients were used for identifying and quantifying Hb by CE. Molecular analysis of α- and β- thalassemia, Hb CS, and Hb Paksé was performed. Results:
Hb CS and Hb Paksé were identified in 573 samples (27.86%) with diverse genotypes. Thirty-eight samples (6.6%) showed no Hb CS peak. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of Hb CS by CE were 93.37, 95.96, 89.92, 97.40, and 95.24%, respectively. The amount of Hb CS in those carrying Hb CS was 0.2-6.5% which showed an increasing trend according to the number of defective α- globin genes, in contrast to Hb A2 levels, which decreased. Hb CS level ≥1.0% accurately excluded heterozygotes and that of ≥2.0% could identify homozygotes. Conclusion: CE has the high potential for identifying and quantifying Hb CS and Hb Paksé, especially in an area with a high prevalence of thalassemia. Hb CS levels can be used as a potential marker to distinguish the genotype of individuals carrying Hb CS.
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Authors | Sitthichai Panyasai, Surada Satthakarn, Amphai Phasit |
Journal | Heliyon
(Heliyon)
Vol. 9
Issue 8
Pg. e19116
(Aug 2023)
ISSN: 2405-8440 [Print] England |
PMID | 37649848
(Publication Type: Journal Article)
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Copyright | © 2023 The Authors. |