Prophylactic effect of chronic immunosuppression in a mouse model of CSF-1 receptor-related leukoencephalopathy.

Abstract

Mutations leading to colony-stimulating factor-1 receptor (CSF-1R) loss-of-function or haploinsufficiency cause CSF1R-related leukoencephalopathy (CRL), an adult-onset disease characterized by loss of myelin and neurodegeneration, for which there is no effective therapy. Symptom onset usually occurs in the fourth decade of life and the penetrance of disease in carriers is high. However, familial studies have identified a few carriers of pathogenic CSF1R mutations that remain asymptomatic even in their seventh decade of life, raising the possibility that the development and severity of disease might be influenced by environmental factors. Here we report new cases in which long-term glucocorticoid treatment is associated with asymptomatic status in elder carriers of pathogenic CSF-1R mutations. The main objective of the present study was to investigate the link between chronic immunosuppression initiated pre-symptomatically and resistance to the development of symptomatic CRL, in the Csf1r+/- mouse model. We show that chronic prednisone administration prevents the development of memory, motor coordination and social interaction deficits, as well as the demyelination, neurodegeneration and microgliosis associated with these deficits. These findings are in agreement with the preliminary clinical observations and support the concept that pre-symptomatic immunosuppression is protective in patients carrying pathogenic CSF1R variants associated with CRL. Proteomic analysis of microglia and oligodendrocytes indicates that prednisone suppresses processes involved in microglial activation and alleviates senescence and improves fitness of oligodendrocytes. This analysis also identifies new potential targets for therapeutic intervention.

• Authors: Violeta Chitu, Fabrizio Biundo, Jude Oppong-Asare, Şölen Gökhan, Jennifer T Aguilan, Jaroslaw Dulski, Zbignew K Wszolek, Simone Sidoli, E Richard Stanley
• Journal: Glia (Glia) Vol. 71 Issue 11 Pg. 2664-2678 (11 2023) ISSN: 1098-1136 [Electronic] United States
• PMID: 37519044 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural)
• Copyright: © 2023 The Authors. GLIA published by Wiley Periodicals LLC.

Chemical References

• Receptor, Macrophage Colony-Stimulating Factor
• Prednisone
• Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
• Receptor Protein-Tyrosine Kinases

Topics

• Mice
• Animals
• Receptor, Macrophage Colony-Stimulating Factor
• Prednisone (pharmacology)
• Proteomics
• Receptors, Granulocyte-Macrophage Colony-Stimulating Factor (genetics)
• Leukoencephalopathies (genetics, prevention & control)
• Microglia
• Receptor Protein-Tyrosine Kinases
• Immunosuppression Therapy