The Antiproliferative Activity of Adiantum pedatum Extract and/or Piceatannol in Phenylhydrazine-Induced Colon Cancer in Male Albino Rats: The miR-145 Expression of the PI-3K/Akt/p53 and Oct4/Sox2/Nanog Pathways.

Abstract

Colon cancer is one of the most common types of cancer worldwide, and its incidence is increasing. Despite advances in medical science, the treatment of colon cancer still poses a significant challenge. This study aimed to investigate the potential protective effects of Adiantum pedatum (AP) extract and/or piceatannol on colon cancer induced via phenylhydrazine (PHZ) in terms of the antioxidant and apoptotic pathways and histopathologic changes in the colons of male albino rats. The rats were randomly divided into eight groups: control, AP extract, piceatannol (P), PHZ, PHZ and AP treatments, PHZ and P treatments, PHZ and both AP and P, and PHZ and prophylaxis with both AP and P. The results demonstrated that PHZ induced oxidative damage, apoptosis, and histopathological changes compared to the control group. However, the administration of AP or P or AP + P as therapy or prophylaxis significantly ameliorated these changes and upregulated the colonic mir-145 and mRNA expression of P53 and PDCD-4 while downregulating the colonic mRNA expression of PI3K, AKT, c-Myc, CK-20, SOX-2, OCT-4, and NanoG compared to the PHZ group. These findings suggest that the candidate drugs may exert their anti-cancer effects through multiple mechanisms, including antioxidant and apoptotic activities.

• Authors: Tarek Khamis, Abd Al-Aziz Abas Diab, Mansour H Zahra, Samih Ebrahim El-Dahmy, Basant Ahmed Abd Al-Hameed, Adel Abdelkhalek, Mahmoud A Said, Hussein Abdellatif, Liana Mihaela Fericean, Ioan Banatean-Dunea, Ahmed Hamed Arisha, Mai S Attia
• Journal: Molecules (Basel, Switzerland) (Molecules) Vol. 28 Issue 14 (Jul 20 2023) ISSN: 1420-3049 [Electronic] Switzerland
• PMID: 37513415 (Publication Type: Journal Article)

Chemical References

• Proto-Oncogene Proteins c-akt
• Tumor Suppressor Protein p53
• 3,3',4,5'-tetrahydroxystilbene
• Antioxidants
• Plant Extracts
• MicroRNAs
• Phenylhydrazines
• RNA, Messenger
• MIRN145 microRNA, rat

Topics

• Rats
• Male
• Animals
• Proto-Oncogene Proteins c-akt (metabolism)
• Tumor Suppressor Protein p53 (genetics)
• Adiantum (metabolism)
• Antioxidants (pharmacology)
• Colonic Neoplasms (chemically induced, drug therapy, metabolism)
• Plant Extracts (pharmacology, therapeutic use)
• MicroRNAs (genetics)
• Phenylhydrazines
• RNA, Messenger