Cancer cell-derived extracellular vesicles (EVs) have unique
protein profiles, making them promising targets as disease
biomarkers. High-grade serous ovarian
carcinoma (HGSOC) is the deadly subtype of
epithelial ovarian cancer, and we aimed to identify HGSOC-specific
membrane proteins. Small EVs (sEVs) and medium/large EVs (m/lEVs) from cell lines or patient serum and
ascites were analyzed by LC-MS/MS, revealing that both EV subtypes had unique proteomic characteristics. Multivalidation steps identified FRĪ±,
Claudin-3, and TACSTD2 as HGSOC-specific sEV
proteins, but m/lEV-associated candidates were not identified. In addition, for using a simple-to-use
microfluidic device for EV isolation, polyketone-coated nanowires (pNWs) were developed, which efficiently purify sEVs from biofluids. Multiplexed array assays of sEVs isolated by pNW showed specific detectability in
cancer patients and predicted clinical status. In summary, the HGSOC-specific marker detection by pNW are a promising platform as clinical
biomarkers, and these insights provide detailed proteomic aspects of diverse EVs in HGSOC patients.