Abstract | BACKGROUND: METHODS: RESULTS: Eight patients (4 MG and 4 SLE) including 5 females and 3 males were involved in this study. The mean age of the patients was 33.13 ± 16.64 years (ranging from 13 to 58 years). The average number of days for mobilization was 7.9 ± 1.6 days, whereas the average number of days for harvesting was 1.5 ± 0.5 days. There was no difference in the number of days for mobilization and harvesting between the MG and SLE groups. The number of CD34+ cells in peripheral blood (PB) on the day of harvesting was 108.37 ± 59.64 x 106 cells/L. There was a significant difference in white blood cell (WBC), neutrophil, monocyte, and platelet cell counts between before and after mobilization. On the day of stem cell harvesting, variables such as WBC, neutrophil, lymphocyte, monocyte, platelet, CD34+ cell counts, and hemoglobin were not different between the MG and SLE groups. The CD34+ recovery percentage following the CD34+ selection procedure was 68.8%, whereas almost 99.9% of the T and B lymphocytes, and NK cells in the PBSC products were eliminated. CONCLUSIONS: Very first attempts in mobilizing, harvesting, and selecting CD34+ stem cells were successful, paving the way for autoimmune patients to have autologous hematopoietic stem cell transplantation in Vietnam.
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Authors | Nguyen Thi Duyen, Mai Van Vien, Ly Tuan Khai, Bui Tien Sy, Phan Quoc Hoan, Le Hai Son, Nguyen Thi Minh Phuong, Le Thi Thu Nga, Ho Xuan Truong, Pham Van Hieu, Tran Thi Huyen Trang, Dao Thi Hong Nga, Nguyen Thanh Binh |
Journal | Clinical laboratory
(Clin Lab)
Vol. 69
Issue 6
(Jun 01 2023)
ISSN: 1433-6510 [Print] Germany |
PMID | 37307119
(Publication Type: Journal Article)
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Chemical References |
- Antigens, CD34
- Cell Adhesion Molecules
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Topics |
- Female
- Male
- Humans
- Adolescent
- Young Adult
- Adult
- Middle Aged
- Leukocytes
- Hematopoietic Stem Cell Transplantation
- Monocytes
- Neutrophils
- Antigens, CD34
- Cell Adhesion Molecules
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