Abstract |
Early antiviral treatments, including intravenous remdesivir (RDV), reduce hospitalization and severe disease caused by COVID-19. An orally bioavailable RDV analog may facilitate earlier treatment of non-hospitalized COVID-19 patients. Here we describe the synthesis and evaluation of alkyl glyceryl ether phosphodiesters of GS-441524 (RVn), lysophospholipid analogs which allow for oral bioavailability and stability in plasma. Oral treatment of SARS-CoV-2-infected BALB/c mice with 1-O-octadecyl-2-O-benzyl-sn-glyceryl-3-phospho-RVn (60 mg/kg orally, once daily for 5 days starting 12h after infection) reduced lung viral load by 1.5 log10 units versus vehicle at day 2 and to below the limit of detection at day 5. Structure/activity evaluation of additional analogs that have hydrophobic ethers at the sn-2 of glycerol revealed improved in vitro antiviral activity by introduction of a 3-fluoro-4-methoxy-substituted benzyl or a 3- or 4-cyano-substituted benzyl. Collectively, our data support the development of RVn phospholipid prodrugs as oral antiviral agents for prevention and treatment of SARS-CoV-2 infections.
|
Authors | Aaron F Carlin, James R Beadle, Alex E Clark, Kendra L Gully, Fernando R Moreira, Ralph S Baric, Rachel L Graham, Nadejda Valiaeva, Sandra L Leibel, William Bray, Rachel E McMillan, Jonathan E Freshman, Aaron F Garretson, Rachael N McVicar, Tariq Rana, Xing-Quan Zhang, Joyce A Murphy, Robert T Schooley, Karl Y Hostetler |
Journal | Journal of medicinal chemistry
(J Med Chem)
Vol. 66
Issue 8
Pg. 5802-5819
(04 27 2023)
ISSN: 1520-4804 [Electronic] United States |
PMID | 37040439
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Antiviral Agents
- Phospholipids
- GS-441524
|
Topics |
- Animals
- Mice
- Antiviral Agents
- SARS-CoV-2
- COVID-19
- Phospholipids
|