As one of the major intractable allergic disorders, atopic
inflammation is commonly accompanied by
itching, dry skin, and
inflammation. Atopic
inflammation deteriorates the quality of life and has no fundamental cure, so it is crucial to urgently explore and develop natural resources for long-term treatment without any side effects. This study aimed to verify Torilis japonica extract (TJE)'s relieving effect and mechanism against atopic
inflammation using skin cells and skin equivalent models, as well as to investigate
torilin's effect (obtained from TJE) and other unknown components as marker compounds.
Torilin concentration was verified in TJE using high-performance liquid chromatography and analyzed the unknown components using nuclear magnetic resonance spectroscopy. Furthermore, TJE's cytotoxicity, regenerative effect, and cell cycle regulation effects were confirmed using skin cells with atopic
inflammation (human dermal fibroblasts and HaCaT keratinocytes) by using TNF-α and IFN-γ treatments. Consequently, TJE was demonstrated to regulate TARC and CTACK expressions as
chemokines and those of
interleukin-4, -5, and -13 as
cytokines related to atopic
inflammation. TJE was further confirmed to affect the
matrix metalloproteinase-1, -2, and -9 expressions, which are essential in skin damage. Lastly, this study confirmed TJE's relieving effect against atopic
inflammation through a 3D skin model and RhCE model using human dermal fibroblasts and HaCaT keratinocytes. These findings on atopic
inflammation verified
torilin's relieving effects and TJE's other components.