Cell Cycle-Related Gene SPC24: A Novel Potential Diagnostic and Prognostic Biomarker for Laryngeal Squamous Cell Cancer.

Abstract

Laryngeal squamous cell cancer (LSCC) is a common malignant tumor with a high degree of malignancy, and its etiology remains unclear. Therefore, screening potential biomarkers is necessary to facilitate the treatment and diagnosis of LSCC. Robust rank aggregation (RRA) analysis was used to integrate two gene expression datasets of LSCC patients from the Gene Expression Omnibus (GEO) database and identify differentially expressed genes (DEGs) between LSCC and nonneoplastic tissues. A gene coexpression network was constructed using weighted gene correlation network analysis (WGCNA) to explore potential associations between the module genes and clinical features of LSCC. Combining differential gene expression analysis and survival analysis, we screened potential hub genes, including CDK1, SPC24, HOXB7, and SELENBP1. Subsequently, western blotting and immunohistochemistry were used to test the protein levels in clinical specimens to verify our findings. Finally, four candidate diagnostic and prognostic biomarkers (CDK1, SPC24, HOXB7, and SELENBP1) were identified. We propose, for the first time, that SPC24 is a gene that may associate with LSCC malignancy and is a novel therapeutic target. These findings may provide important mechanistic insight of LSCC.

• Authors: Jialei Chen, Jing Luo, Jing He, Xianyao Jiang, Ning Jiang, Changhong Yang, Shixun Zhong
• Journal: BioMed research international (Biomed Res Int) Vol. 2023 Pg. 1733100 ( 2023) ISSN: 2314-6141 [Electronic] United States
• PMID: 36718148 (Publication Type: Journal Article)
• Copyright: Copyright © 2023 Jialei Chen et al.

Chemical References

• Biomarkers, Tumor
• SPC24 protein, human
• Microtubule-Associated Proteins
• HOXB7 protein, human
• Homeodomain Proteins

Topics

• Humans
• Laryngeal Neoplasms (diagnosis, genetics, metabolism)
• Prognosis
• Squamous Cell Carcinoma of Head and Neck (diagnosis, genetics)
• Head and Neck Neoplasms (genetics)
• Cell Cycle (genetics)
• Neoplasms, Squamous Cell
• Biomarkers, Tumor (genetics, metabolism)
• Gene Expression Regulation, Neoplastic (genetics)
• Microtubule-Associated Proteins (genetics)
• Homeodomain Proteins (genetics)