Assessment the Effect of Human Umbilical Cord Wharton's Jelly Stem Cells on the Expression of Homing Genes: CXCR4 and VLA-4 in Cell Line of Prostate Cancer.

Abstract

Background : Prostate cancer is the second most common cancer in the male that affects the health, social and economic life of person. Different compounds such as Wharton's jelly, have been used to treat prostate cancer. Wharton's jelly is a tissue rich in cells with mesenchymal morphology. Wharton's jelly compound inhibited the growth of various cancer cells, including ovarian, osteosarcoma, breast, and prostate cancers, and also reduced the expression of CXCR4 and VLA-4 genes involved in the metastasis process. Materials and Methods: To do this research, Wharton's jelly stem cells and DU145 cancer cell line were cultured. After cell culture, the effect of Wharton's jelly on this cell line was evaluated by scratching and MTT assay. The expression of CXCR4 and VLA-4 genes was also evaluated by Real-time PCR. Results: The results of MTT and Scratching tests showed that Wharton's jelly inhibited the growth of DU145 cancer cells and also decreased the expression level of CXCR4 and VLA-4 genes. Conclusion: The results of this study showed that Wharton's jelly can be considered as an effective compound for decreasing metastasis of prostate cancer.

• Authors: Roya Asl Iranifam, Masoud Maleki, Vida Vahdani Kia, Esmaeil Safavi, Negin Sadi Khosroshahi
• Journal: International journal of hematology-oncology and stem cell research (Int J Hematol Oncol Stem Cell Res) Vol. 16 Issue 3 Pg. 157-163 (Jul 01 2022) ISSN: 2008-3009 [Print] Iran
• PMID: 36694702 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 Tehran University of Medical Sciences.