Therapeutic efficacy of n-Docosanol against velogenic Newcastle disease virus infection in domestic chickens.

Abstract

Introduction:

The control of Newcastle disease virus (NDV) infection depends solely on vaccination which in most cases is not sufficient to restrain the consequences of such a highly evolving viral disease. Finding out substances for preparing an efficient anti-ND drug would be of high value. n-Docosanol is a saturated fatty alcohol with an inhibitory effect against many enveloped viruses. In this study, we evaluated the therapeutic effect of n-docosanol on NDV infection and shedding in chickens.

Methods:

Chickens infected with a highly virulent NDV were treated with low to high concentrations of n-docosanol (20, 40, and 60 mg/kg body weight) for 4-successive days, once they showed the disease symptoms. Survival and curative rates, virus load, histopathological scoring, and virus shedding were defined.

Results:

Symptoms development was found to discontinue 24-72 hours post-treatment. Survival rate in the NDV-infected chickens raised 37.4-53.2% after the treatment. n-Docosanol treatment was also found to significantly reduce virus load in the digestive (26.2-33.9%), respiratory (38.3-63%), nervous (26.7-51.1%), and lymphatic (16.4-29.1%) tissues. Histopathological scoring of NDV lesions revealed prominent rescue effects on the histology of different tissues. Importantly, n-docosanol treatment significantly reduced virus shedding in oropharyngeal discharge and feces thereby allowing the restriction of NDV spread.

Conclusion:

Our findings suggest n-docosanol as a promising remedy in the control strategy of Newcastle disease in the poultry industry.

• Authors: Ahmed Orabi, Ashraf Hussein, Ayman A Saleh, Ayman M Megahed, Mohamed Metwally, Hassan Moeini, Aya Sh Metwally
• Journal: Frontiers in microbiology (Front Microbiol) Vol. 13 Pg. 1049037 ( 2022) ISSN: 1664-302X [Print] Switzerland
• PMID: 36483200 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 Orabi, Hussein, Saleh, Megahed, Metwally, Moeini and Metwally.