Separation of two isozymes of polyamine oxidase from murine L1210 leukemia cells.

Abstract

Polyamine oxidase from L1210 cells has been separated into two isozymes, A and B on the basis of chromatography on hydroxyapatite. The two isozymes also show different elution characteristics from MGBG Sepharose and Sephacryl-300. The molecular weights of A and B isozymes are estimated to be 260,000 and 200,000 daltons, respectively. The two isozymes show different kinetic characteristics with spermidine, spermine, N1-acetylspermine, and N1,N12-diacetylspermine.

• Authors: P R Libby, C W Porter
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 144 Issue 1 Pg. 528-35 (Apr 14 1987) ISSN: 0006-291X [Print] United States
• PMID: 3579924 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Isoenzymes
• N'-acetylspermine
• Spermine
• N',N''-diacetylspermine
• Oxidoreductases Acting on CH-NH Group Donors

Topics

• Animals
• Isoenzymes (isolation & purification, metabolism)
• Kinetics
• Leukemia L1210 (enzymology)
• Mice
• Mice, Inbred DBA
• Molecular Weight
• Oxidoreductases Acting on CH-NH Group Donors (isolation & purification, metabolism)
• Spermine (analogs & derivatives)
• Substrate Specificity
• Polyamine Oxidase