Abstract |
The long-term treatment of glucocorticoids is a common cause of osteoporosis (OP). This study concentrated on inquiring into the regulatory role and potential mechanisms of TRG-AS1 on dexamethasone (Dex)-induced OP in rats. We adopted Dex to treat rat osteoblasts and rats to simulate in-vitro and in-vivo OP models, respectively. Gain-of-function assays of TRG-AS1, miR-802 and CAB39 were constructed in rat osteoblasts to make certain the influence of TRG-AS1, miR-802 and CAB39 on differentiation, proliferation and apoptosis of rat osteoblasts. TRG-AS1 and CAB39 were down-regulated in the Dex-induced OP model in rats, in contrast to miR-802. Overexpression of TRG-AS1 restrained Dex-induced inhibition of osteogenic differentiation, promoted CAB39/AMPK/ SIRT-1 and inhibited NF-κB, while overexpression of miR-802 bridled the inhibitory effect of TRG-AS1 on OP. miR-802 was targeted by TRG-AS1, and inhibited CAB39. Inhibition of either AMPK or SIRT-1 abated the osteogenic differentiation-promoting effect of CAB39. Animal experiments displayed that overexpressing TRG-AS1 alleviated Dex-induced OP in rats. In conclusion, up-regulation of TRG-AS1 protected against glucocorticoid-induced OP in rats by modulating the miR-802-mediated CAB39/AMPK/ SIRT-1/NF-κB axis.
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Authors | Wen Liu, Guojuan Li, Jing Li, Wei Chen |
Journal | Human cell
(Hum Cell)
Vol. 35
Issue 5
Pg. 1424-1439
(Sep 2022)
ISSN: 1749-0774 [Electronic] Japan |
PMID | 35794445
(Publication Type: Journal Article)
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Copyright | © 2022. The Author(s) under exclusive licence to Japan Human Cell Society. |
Chemical References |
- Cab39 protein, rat
- Calcium-Binding Proteins
- Glucocorticoids
- MicroRNAs
- NF-kappa B
- RNA, Long Noncoding
- AMP-Activated Protein Kinases
- Sirt1 protein, rat
- Sirtuin 1
- Sirtuins
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Topics |
- AMP-Activated Protein Kinases
(genetics)
- Animals
- Apoptosis
(genetics)
- Calcium-Binding Proteins
- Glucocorticoids
(adverse effects)
- MicroRNAs
(genetics)
- NF-kappa B
- Osteogenesis
(genetics)
- Osteoporosis
(chemically induced, genetics, prevention & control)
- RNA, Long Noncoding
(genetics)
- Rats
- Sirtuin 1
- Sirtuins
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