Abstract | Background: Materials and Methods: From 2001 to 2015, we retrospectively collected 120 tissue samples from consecutive Tibetan GC patients and constructed tissue microarrays. EBV infection was assessed by Epstein-Barr-encoded RNA (EBER) in situ hybridization, and MMR protein levels were measured. Immune markers (including CD3 and CD8) in intraepithelial, stromal, and total areas were detected by immunohistochemistry (IHC). PD-L1 expression was assessed by the combined positive score (CPS). We also analyzed the relationships of EBV infection and MMR status with immune markers. Results: Of the 120 samples, 11 (9.17%) were EBV positive (+), and 6 (5%) were MMR deficient (dMMR). PD-L1 CPS ≥1% was found in 32.5% (39/120) of Tibetan GC patients. EBV infection was associated with higher numbers of CD3+ T cells (P < 0.05) and CD8+ T cells (P < 0.05) and higher PD-L1 expression (P < 0.05). For the limited number of dMMR patients, no significant relationship was observed between dMMR and TIME markers (P > 0.05). Conclusions: In Tibetan GC patients, the rates of EBV infection, dMMR, and positive PD-L1 expression were 9.17%, 5%, and 32.5%, respectively. EBV infection was associated with the numbers of CD3+ T cells and CD8+ T cells and PD-L1 expression within the tumor. These markers may guide the selection of Tibetan GC patients for immunotherapy.
|
Authors | Jie Shi, Xu Yang, Xinmei Wang, Yufeng Luo, Weixun Zhou, Hanhuan Luo, Zhaxi Bianba, Zhuoma Nima, Qian Wang, Han Wang, Ruiqian Liao, Quzhen Ciren, Mei Li, Junyi Pang |
Journal | BioMed research international
(Biomed Res Int)
Vol. 2022
Pg. 2684065
( 2022)
ISSN: 2314-6141 [Electronic] United States |
PMID | 35734348
(Publication Type: Journal Article)
|
Copyright | Copyright © 2022 Jie Shi et al. |
Chemical References |
- B7-H1 Antigen
- Biomarkers, Tumor
- Immune Checkpoint Inhibitors
- Programmed Cell Death 1 Receptor
|
Topics |
- B7-H1 Antigen
(metabolism)
- Biomarkers, Tumor
(genetics)
- Brain Neoplasms
- Colorectal Neoplasms
- DNA Mismatch Repair
(genetics)
- Epstein-Barr Virus Infections
(complications, epidemiology)
- Herpesvirus 4, Human
(genetics, metabolism)
- Humans
- Immune Checkpoint Inhibitors
- Neoplastic Syndromes, Hereditary
- Prevalence
- Programmed Cell Death 1 Receptor
(genetics)
- Protein Deficiency
(complications)
- Retrospective Studies
- Stomach Neoplasms
(epidemiology, genetics, metabolism)
- Tibet
(epidemiology)
- Tumor Microenvironment
(genetics)
|