Diffuse midline glioma with H3-K27M mutation: A rare case with GFAP-positive anucleate whorled patterns.

Abstract

INTRODUCTION:

Diffuse midline glioma with H3-K27M mutation is an infiltrative high-grade glioma, with predominantly astrocytic differentiation.

PATIENT CONCERNS:

A 54-year-old Chinese woman presented with memory loss for a month and walking instability for 15 days.

DIAGNOSIS:

Magnetic resonance imaging showed a mass shadow of isometric T1 and slightly longer T2 with mild mixed signals in the third ventricle of the suprasellar region. Histologically, the tumor was primarily sheet-like, with many "anucleate areas" composed of long and thin fibrillary processes of the bipolar cells, which formed "whorls." The neoplastic nuclei were ovoid and moderate in size. The tumor showed brisk mitotic activity and vascular proliferation, with no necrosis. In addition to histone H3K27M mutation, immunohistochemical staining showed that the tumor cells were positive for glial fibrillary acidic protein, oligodendrocyte transcription factor 2, alpha-thalassemia/mental retardation syndrome X, S-100 and Vimentin. The "anucleate areas" were positive for glial fibrillary acidic protein and negative for synaptophysin. The Ki-67 proliferation index was about 10%. Molecular genetic analyses detected H3F3A K27M mutation, but no mutations in IDH1 or IDH2, TERT promoter mutations, MGMT promoter methylation, KIAA1549-BRAF fusion or deletion of 1p/19q were found. Based on these findings, the patient was diagnosed as diffuse midline glioma with H3-K27M mutation in the third ventricle, corresponding to WHO grade 4.

INTERVENTIONS:

A craniotomy with total excision of the tumor was performed.

OUTCOMES:

After surgery, she was routinely treated with temozolomide for chemotherapy and synchronous radiotherapy. It has been 11 months now, and the patient is living well.

CONCLUSION:

This case report provides information on the microscopic morphological features of diffuse midline glioma with H3K27M mutation, which can help pathologists to make a definitive diagnosis of this tumor.

• Authors: Yi-Hua Wang, Jian Gu, Juan-Han Yu, Lin Fu, Qing-Chang Li, Xue-Shan Qiu, En-Hua Wang
• Journal: Medicine (Medicine (Baltimore)) Vol. 101 Issue 24 Pg. e29448 (Jun 17 2022) ISSN: 1536-5964 [Electronic] United States
• PMID: 35713454 (Publication Type: Case Reports, Journal Article)
• Copyright: Copyright © 2022 the Author(s). Published by Wolters Kluwer Health, Inc.

Chemical References

• Glial Fibrillary Acidic Protein
• Histones

Topics

• Brain Neoplasms (pathology)
• Female
• Glial Fibrillary Acidic Protein (genetics)
• Glioma (diagnosis, genetics, metabolism)
• Histones (genetics)
• Humans
• Mutation