Intracerebroventricular injection of taurine induces hypothermia through modifying monoaminergic pathways in chicks.

Abstract

Brain monoamines are reported to regulate body temperature and food intake. The objective of this study was to investigate the mechanism of brain monoamine metabolism in taurine-induced hypothermia and appetite suppression. In Experiment 1, 5-day-old male Julia layer chicks (n = 10) were subjected to intracerebroventricular (ICV) injection with saline or taurine (5 μmol/10 μL). In Experiment 2, the chicks were ICV injected with saline, taurine, fusaric acid (dopamine-β-hydroxylase inhibitor: 558 nmol), or taurine with fusaric acid. In Experiment 3, the chicks were ICV injected with saline, taurine, para-chlorophenylalanine (PCPA, tryptophan hydroxylase inhibitor: 400 nmol), or taurine with PCPA. In Experiment 4, the chicks were ICV injected with saline, taurine, clorgyline (monoamine oxidase inhibitor: 81 nmol), or taurine with clorgyline. Central taurine lowered rectal temperature at 30 min post-injection and increased norepinephrine in the brainstem and its metabolite 3-methoxy-4-hydroxyphenylglycol in both the diencephalon and brainstem. Similarly, taurine treatment induced increases in serotonin (5-HT) and its metabolite 5-hydroxyindoleacetic acid in the diencephalon. Fusaric acid completely and PCPA partially, but not clorgyline, attenuated taurine-induced hypothermia. The anorexigenic effect of taurine was partially attenuated by PCPA, but not fusaric acid nor clorgyline. In conclusion, central taurine activates dopamine-β-hydroxylase and tryptophan hydroxylase to produce norepinephrine and 5-HT, and then induces hypothermia, but 5-HT alone may be linked with taurine-induced anorexia in chicks.

• Authors: Mohamed Z Elhussiny, Haruka Nishimura, Phuong V Tran, Shogo Haraguchi, Elizabeth R Gilbert, Mark A Cline, Takashi Bungo, Mitsuhiro Furuse, Vishwajit S Chowdhury
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 928 Pg. 175092 (Aug 05 2022) ISSN: 1879-0712 [Electronic] Netherlands
• PMID: 35697149 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 Elsevier B.V. All rights reserved.

Chemical References

• Taurine
• Serotonin
• Tryptophan Hydroxylase
• Fenclonine
• Dopamine
• Norepinephrine

Topics

• Animals
• Chickens (metabolism)
• Dopamine (pharmacology)
• Eating
• Fenclonine (pharmacology)
• Hypothermia (chemically induced)
• Male
• Norepinephrine (pharmacology)
• Serotonin (metabolism)
• Taurine (pharmacology)
• Tryptophan Hydroxylase (pharmacology)