Long non-coding RNA BBOX1-antisense RNA 1 enhances cell proliferation and migration and suppresses apoptosis in oral squamous cell carcinoma via the miR-3940-3p/laminin subunit gamma 2 axis.

Abstract	Long non-coding RNAs (lncRNAs) play an essential role in oral squamous cell carcinoma (OSCC). We aimed to demonstrate the effects of lncRNA gamma-butyrobetaine hydroxylase 1 (BBOX1)-antisense RNA 1 (AS1) in OSCC and its regulatory mechanisms. The levels of BBOX1-AS1, microRNA (miR)-3940-3p, and laminin subunit gamma 2 (LAMC2) in OSCC were determined using reverse transcription-quantitative polymerase chain reaction. The correlations among BBOX1-AS1, miR-3940-3p, and LAMC2 were validated using luciferase, pull-down, and RNA immunoprecipitation assays. Cell proliferation, migration, and apoptosis were examined. BBOX1-AS1 and LAMC2 were notably overexpressed in OSCC, while miR-3940-3p showed the opposite trend. BBOX-1-AS1 silencing reduced the cell proliferation and migration, while promoting apoptosis. Mechanistically, BBOX1-AS1 modulates LAMC2 expression by competitively binding to miR-3940-3p. miR-3940-3p inhibition alleviated the inhibitory effects of BBOX1-AS1 deficiency on OSCC development. LAMC2 knockdown reversed these changes. Our results revealed that BBOX1-AS1 promotes the malignant phenotype of OSCC cells via the upregulation of LAMC2 expression by targeting miR-3940-3p, indicating that BBOX1-AS1 may be a novel target for OSCC intervention.
Authors	Chunguang Zhao, Wei Shi, Min Chen
Journal	Bioengineered (Bioengineered) Vol. 13 Issue 4 Pg. 11138-11153 (04 2022) ISSN: 2165-5987 [Electronic] United States
PMID	35506252 (Publication Type: Journal Article)
Chemical References	LAMC2 protein, human Laminin MIRN3940 microRNA, human MicroRNAs RNA, Antisense RNA, Long Noncoding BBOX1 protein, human gamma-Butyrobetaine Dioxygenase
Topics	Apoptosis (genetics) Carcinoma, Squamous Cell (genetics, metabolism, pathology) Cell Movement Cell Proliferation Head and Neck Neoplasms Humans Laminin (metabolism) MicroRNAs (genetics, metabolism) Mouth Neoplasms (genetics, pathology) RNA, Antisense RNA, Long Noncoding (genetics, metabolism) Squamous Cell Carcinoma of Head and Neck gamma-Butyrobetaine Dioxygenase (metabolism)

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