Abstract | Background: Methods: In a pilot case-control study, we enrolled 19 ACS patients (within 72-h of cardiac events) and 19 healthy-controls. Gut barrier function was determined using lactulose-to- mannitol urinary excretion ratio (L/M ratio). Stool microbiome composition was examined using16S sequencing and predictive functional analysis for LPS biosynthesis pathway by PICRUSt tool. Serum TMAO and LPS levels were measured. Results: ACS patients had increased Gammaproteobacteria compared to controls:1.8 ±3.0 vs. 0.2 ±0.4% (P =0.04). Though Proteobacteria level was increased but not statistically significant: 4.1 ±3.8 vs. 2.1 ±1.7% (P =0.056). L/M-ratio was three times higher in ACS patients; 0.06 ±0.07 vs 0.023 ±0.02, (P =0.014). Surprisingly, there was no difference in the mean serum LPS or TMAO levels. However, PICRUSt analysis indicated increased Proteobacteria population increasingly contributed to LPS biosynthesis in ACS patients only. Conclusions: ACS patients likely to have leaky-gut and perturbed gut microbiota. Further studies are required to precisely define the role of dysbiosis in ACS.
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Authors | Tarik Alhmoud, Anand Kumar, Chien-Chi Lo, Rana Al-Sadi, Stacey Clegg, Ihab Alomari, Tarek Zmeili, Cheryl Diane Gleasne, Kim Mcmurry, Armand Earl Ko Dichosa, Momchilo Vuyisich, Patrick Sam Guy Chain, Shiraz Mishra, Thomas Ma |
Journal | Human microbiome journal
(Hum Microb J)
Vol. 13
(Aug 2019)
ISSN: 2452-2317 [Electronic] England |
PMID | 35506046
(Publication Type: Journal Article)
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