Interaction between AhR and HIF-1 signaling pathways mediated by ARNT/HIF-1β.

Abstract

BACKGROUND:

The main causes of lung cancer are smoking, environmental pollution and genetic susceptibility. It is an indisputable fact that PAHs are related to lung cancer, and benzo(a) pyrene is a representative of PAHs. The purpose of the current investigation was to investigate the interaction between AhR and HIF-1 signaling pathways in A549 cells, which provide some experimental basis for scientists to find drugs that block AhR and HIF-1 signaling pathway to prevent and treat cancer.

METHODS:

This project adopts the CYP1A1 signaling pathways and the expression of CYP1B1 is expressed as a measure of AhR strength index. The expression of VEGF and CAIX volume as a measure of the strength of the signal path HIF-1 indicators. Through the construction of plasmid vector, fluorescence resonance energy transfer, real-time quantitative PCR, western blotting and immunoprecipitation, the interaction between AhR signaling pathway and HIF-1 signaling pathway was observed.

RESULTS:

BaP can enhance the binding ability of HIF-1α protein to HIF-1β/ARNT in a dose-dependent manner without CoCl2. However, the binding ability of AhR protein to HIF-1β/ARNT is inhibited by HIF-1α signaling pathway in a dose-dependent manner with CoCl2.

CONCLUSION:

It is shown that activation of the AhR signaling pathway does not inhibit the HIF-1α signaling pathway, but activation of the HIF-1α signaling pathway inhibits the AhR signaling pathway.

• Authors: Mengdi Zhang, Yuxia Hu, Fan Yang, Jingwen Zhang, Jianxin Zhang, Wanjia Yu, Minjie Wang, Xiaoli Lv, Jun Li, Tuya Bai, Fuhou Chang
• Journal: BMC pharmacology & toxicology (BMC Pharmacol Toxicol) Vol. 23 Issue 1 Pg. 26 (04 26 2022) ISSN: 2050-6511 [Electronic] England
• PMID: 35473600 (Publication Type: Journal Article)
• Copyright: © 2022. The Author(s).

Chemical References

• ARNT protein, human
• Receptors, Aryl Hydrocarbon
• Aryl Hydrocarbon Receptor Nuclear Translocator

Topics

• A549 Cells
• Aryl Hydrocarbon Receptor Nuclear Translocator (genetics, metabolism)
• Humans
• Lung Neoplasms (metabolism)
• Receptors, Aryl Hydrocarbon (genetics, metabolism)
• Signal Transduction