Abstract | Background: There is growing evidence that long non-coding RNAs (LncRNAs) are key in the development of a variety of human tumors. However, the role of lncRNA GTF2IRD2P1 has not been well studied in cancer. The impact of GTF2IRD2P1 on the biological function and clinical relevance in bladder cancer is largely unknown. This study aimed to investigate the biological role of GTF2IRD2P1 in bladder evolution and carcinogenesis. Methods: Results: GTF2IRD2P1 expression was found to be lower in both human bladder cancer tissues and cell lines (UM-UC-3, RT4, and 5637), and elevated in T24 compared to the corresponding normal controls. GTF2IRD2P1 expression was also enhanced after transfection of UM-UC-3 cells with the overexpression vector. Meanwhile, overexpression of GTF2IRD2P1 inhibited the proliferation of UM-UC-3 and prolonged the cell cycle. The silencing of GTF2IRD2P1 significantly increased the proliferation and shortened the cell cycle of T24 cells and induced Wnt signaling activity to promote the progression of bladder cancer. Similarly, the transplanted tumor nude mouse model demonstrated that silencing GTF2IRD2P1 strengthens the progression of bladder cancer by targeting the Wnt signaling pathway.
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Authors | Zhuo Huang, Hongbin Gao, Liangliang Qing, Biao Wang, Chaoyong He, Ning Luo, Chuncheng Lu, Shipeng Fan, Peng Gu, Hui Zhao |
Journal | PeerJ
(PeerJ)
Vol. 10
Pg. e13220
( 2022)
ISSN: 2167-8359 [Print] United States |
PMID | 35433119
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2022 Huang et al. |
Chemical References |
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Topics |
- Animals
- Mice
- Humans
- RNA, Long Noncoding
(genetics)
- Wnt Signaling Pathway
(genetics)
- Cell Line, Tumor
- Carcinoma, Transitional Cell
- Mice, Nude
- Urinary Bladder Neoplasms
(genetics)
- Cell Proliferation
(genetics)
- Carcinogenesis
(genetics)
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