Abstract |
In this study, a novel low molecular weight polysaccharide (named LMW-BSP) was extracted from Bletilla striata at 4 °C. The results of structural characteristics analysis showed that LMW-BSP was a 23 kDa neutral polysaccharide contained glucose and mannose at a molar ratio of 1.00:1.26. Structural investigations of the periodate oxidation studies, Smith-degradation as well as methylation were performed, and combined with 1D and 2D NMR spectroscopy, the main chain residues sequence of LMW-BSP was concluded to be: α-D-Manp-(1 → 3)-β-D-Manp-(1 → [4)-β-D-Glcp-(1]2 → 4)-β-D-Manp-(1 → 3)-β-D-Manp-(1→. Moreover, the antitumor activity of LMW-BSP was evaluated in H22 tumor-bearing mice. And the results suggested that LMW-BSP could effectively improve immune cells activities and lymphocytes subsets proportions dose-dependently in tumor-bearing mice, leading to the apoptosis of H22 cells via G1 phase arrested. LMW-BSP inhibited tumor growth and exhibited antitumor effects in vivo. And it supported considering the novel polysaccharide as a potential drug component in hepatocellular carcinoma treatment.
|
Authors | Chao Liu, Ke-Yao Dai, Hai-Yu Ji, Xiao-Yu Jia, An-Jun Liu |
Journal | International journal of biological macromolecules
(Int J Biol Macromol)
Vol. 205
Pg. 553-562
(Apr 30 2022)
ISSN: 1879-0003 [Electronic] Netherlands |
PMID | 35202634
(Publication Type: Journal Article)
|
Copyright | Copyright © 2022 Elsevier B.V. All rights reserved. |
Chemical References |
|
Topics |
- Animals
- Mannose
- Mice
- Molecular Weight
- Neoplasms
- Orchidaceae
(chemistry)
- Polysaccharides
(chemistry, pharmacology)
|