Abstract |
The burden of breast cancer (BC) has exacerbated over decades. Paclitaxel resistance is responsible for increasing BC treatment burden. Nuclear receptor binding SET domain-containing protein 1 (NSD1) is positively correlated with a poor prognosis in patients with BC. This study investigates the function of NSD1 in paclitaxel-resistant (PR) BC cells. The high levels of NSD1 and Wnt10b in PR BC cell lines (MCF-7/PR) or MCF-7 parental cells were determined by RT-qPCR. Western blotting was conducted to measure the levels of NSD1 protein, apoptosis-associated proteins, Wnt10b protein, H3K36me2 protein, H3K27me3 protein, and signal pathway-associated proteins in MCF-7/PR cells or MCF-7 cells or in vivo subcutaneous xenografted tumor model, and the results demonstrated that NSD1 inhibited cell apoptosis and promoted cell proliferation and tumor growth via activating Wnt/β- catenin pathway. Cell apoptosis and viability were estimated using cell counting kit-8 assays and flow cytometry. Positive correlation between NSD1 and Wnt10b was identified by chromatin immunoprecipitation assay. The distribution of β- catenin was determined by immunofluorescence assays. We conclude that NSD1 knockdown inhibits the viability and promotes the apoptosis of paclitaxel-resistant BC cells by inactivating the NSD1/H3K27me3/Wnt10b/β- catenin signaling pathway.
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Authors | Yi Chen, Xiao Li, Jin Xu, Hua Xiao, Cuiju Tang, Wei Liang, Xuedan Zhu, Yueyu Fang, Hanjin Wang, Junfeng Shi |
Journal | Bioengineered
(Bioengineered)
Vol. 13
Issue 2
Pg. 3526-3536
(02 2022)
ISSN: 2165-5987 [Electronic] United States |
PMID | 35200072
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- beta Catenin
- Histone-Lysine N-Methyltransferase
- NSD1 protein, human
- Paclitaxel
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Topics |
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects)
- Breast Neoplasms
(drug therapy, genetics, metabolism, physiopathology)
- Cell Proliferation
(drug effects)
- Drug Resistance, Neoplasm
- Female
- Histone-Lysine N-Methyltransferase
(genetics, metabolism)
- Humans
- MCF-7 Cells
- Paclitaxel
(pharmacology)
- Wnt Signaling Pathway
(drug effects)
- beta Catenin
(genetics, metabolism)
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