Dual-molecular targeted NIR II probe with enhanced response for head and neck squamous cell carcinoma imaging.

Abstract

In this research, a fluorescent probe of 7-(diethylamine) coumarin derivatives with multiple binding sites to detect biothiols in tumor cell with strong NIR II luminescencein vivowas synthesized. The biothiols include cysteine (Cys) and glutathione (GSH) in tumor cells, and the tumor-response luminescence was proved by the cell experiment. Importantly, the monolayer functional phospholipid (DSPE-PEG) coating and aggregation induced emission (AIE) dye of TPE modification made the probe have good stability and biocompatibility with little luminescence quenching in aqueous phase, which was proved byin vitroandin vivoexperiments. The final aqueous NIR II probe combined with bevacizumab (for VEGF recognition in the cancer cells) and Capmatinib (for Met protein recognition in the cancer cells) has stronger targeted imaging on head and neck squamous cell carcinoma (HNSCC) cancer with intravenous injection. This GSH/Cys detection in the tumor cell and strong dual-molecular NIR II bioimagingin vivomay provide new strategy to tumor detection.

• Authors: Yaqun Ma, Yanxing Wang, Xin Hui, Bi Lin, Ying Yuan, Xiaofeng Tao, Ruichan Lv
• Journal: Nanotechnology (Nanotechnology) Vol. 33 Issue 22 (Mar 09 2022) ISSN: 1361-6528 [Electronic] England
• PMID: 35189605 (Publication Type: Journal Article)
• Copyright: © 2022 IOP Publishing Ltd.

Chemical References

• Fluorescent Dyes
• Molecular Probes
• Glutathione
• Cysteine

Topics

• Cell Line, Tumor
• Cysteine (metabolism)
• Diagnostic Imaging (methods)
• Fluorescent Dyes (metabolism)
• Glutathione (metabolism)
• Head and Neck Neoplasms (diagnostic imaging, metabolism)
• Humans
• Luminescence
• Molecular Probes (metabolism)
• Molecular Targeted Therapy (methods)
• Squamous Cell Carcinoma of Head and Neck (diagnostic imaging, metabolism)