β-
Elemene is an effective anti-
cancer ingredient extracted from the genus Curcuma (Zingiberaceae familiy). In the present study, we demonstrated that β-
elemene inhibited the proliferation of
colorectal cancer cells and induced cell cycle arrest in the G2/M phase. In addition, β-
elemene induced nuclear
chromatin condensation and cell membrane
phosphatidylserine eversion, decreased cell mitochondrial membrane potential, and promoted the cleavage of
caspase-3,
caspase-9 and PARP
proteins, indicating apoptosis in
colorectal cancer cells. At the same time, β-
elemene induced autophagy response, and the treated cells showed autophagic vesicle bilayer membrane structure, which was accompanied by up-regulation of the expression of LC3B and SQSTM1. Furthermore, β-
elemene increased ROS levels in
colorectal cancer cells, promoted phosphorylation of AMPK
protein, and inhibited
mTOR protein phosphorylation. In the experiments in vivo, β-
elemene inhibited the
tumor size and induced apoptosis and autophagy in nude mice. In summary, β-
elemene inhibited the occurrence and development of
colon cancer xenografts in nude mice, and significantly induced apoptosis and autophagy in
colorectal cancer cells in vitro. These effects were associated with regulation of the ROS/AMPK/mTOR signaling. We offered a molecular basis for the development of β-
elemene as a promising anti-
tumor drug candidate for
colorectal cancer.