Abstract | BACKGROUND: PURPOSE: METHOD: TNBS 40 mg/kg was utilized to establish UC model. Rats were sacrificed after gavage for 7 days. Body weight loss, disease activity index (DAI), colonic mucosal damage index (CMDI) and histopathology were measured. Intestinal content samples were collected, and analyzed by 16 S rRNA sequencing. Western blot, immunohistochemistry and real-time polymerase chain reaction were used to evaluate the regulation mechanism of HL+HP in UC model rats. RESULTS: The results showed that the DAI score, CMDI score and histological score were significantly decreased in each group. The symptoms of diarrhea, hematochezia, colonic mucosal injury and congestion and edema were improved. Sequencing results of intestinal flora showed that the abundance of probiotics such as Akkermansia and Blautia was increased in HL group and HL+HP group, while probiotics such as Allobaculum and Alloprevotella were increased in HP group. The intestinal pathogenic bacteria such as Escherichia-Shigella and Clostridium_sensu_stricto_1 were decreased. In addition, HL+HP could also inhibit the inflammatory response and protect the integrity of the tight junction to play an anti-UC effect. CONCLUSION: Coptis chinensis Franch and Magnolia officinalis might prevent intestinal barrier damage by regulating intestinal flora imbalance and inhibit the inflammatory response.
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Authors | Qian Xie, Hongyan Li, Rong Ma, Mihong Ren, Yong Li, Jinxiu Li, Hai Chen, Zhuoping Chen, Daoyin Gong, Jian Wang |
Journal | Phytomedicine : international journal of phytotherapy and phytopharmacology
(Phytomedicine)
Vol. 97
Pg. 153927
(Mar 2022)
ISSN: 1618-095X [Electronic] Germany |
PMID | 35030387
(Publication Type: Journal Article)
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Copyright | Copyright © 2022 Elsevier GmbH. All rights reserved. |
Chemical References |
- Trinitrobenzenesulfonic Acid
|
Topics |
- Animals
- Colitis, Ulcerative
(chemically induced, drug therapy)
- Colon
- Coptis chinensis
- Disease Models, Animal
- Gastrointestinal Microbiome
- Magnolia
- Rats
- Trinitrobenzenesulfonic Acid
|