MicroRNA-603 Promotes Progression of Cutaneous Melanoma by Regulating TBX5.

Abstract

BACKGROUND:

Although studies manifested that microRNA-603 plays a vital role in many cancers, the modulatory mechanism of microRNA-603 in cutaneous melanoma remains unknown. We aimed to investigate the roles of microRNA-603 in cutaneous melanoma cells.

METHODS:

First, microRNA-603 expression in cutaneous melanoma cell lines was detected by qRT-PCR. The mRNA and protein expression levels of TBX5 in cutaneous melanoma cell lines were tested by qRT-PCR and western blot, respectively. In addition, the interaction between microRNA-603 and TBX5 was determined by dual-luciferase reporter gene assay, and their impacts on the growth of cutaneous melanoma cells were detected by cellular function experiments such as MTT, colony formation, and Transwell assays.

RESULTS:

The expression level of microRNA-603 in human cutaneous melanoma cells was relatively upregulated. Overexpressing microRNA-603 could promote progression of cutaneous melanoma cells, while silencing microRNA-603 expression could suppress the malignant progression of cutaneous melanoma. In addition, TBX5 was lowly expressed in cutaneous melanoma cells. As confirmed by dual-luciferase assay, microRNA-603 could specifically bind to 3'UTR of TBX5 and regulate TBX5. The results of the rescue experiment demonstrated that inhibiting microRNA-603 expression could suppress the proliferation, migration, and invasion of cutaneous melanoma cells, but its suppressive effect could be restored by TBX5.

CONCLUSION:

MicroRNA-603 could regulate the expression of TBX5, thus promoting the malignant progression of cutaneous melanoma cells.

• Authors: Xianghua Dong, Ying Wang, Yan Qu, Junru Liu, Xien Feng, Xuechao Xu
• Journal: Computational and mathematical methods in medicine (Comput Math Methods Med) Vol. 2021 Pg. 1888501 ( 2021) ISSN: 1748-6718 [Electronic] United States
• PMID: 35003317 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 Xianghua Dong et al.

Chemical References

• 3' Untranslated Regions
• MIRN603 microRNA, human
• MicroRNAs
• T-Box Domain Proteins
• T-box transcription factor 5

Topics

• 3' Untranslated Regions
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Computational Biology
• Disease Progression
• Down-Regulation
• Gene Expression Regulation, Neoplastic
• Humans
• Melanoma (genetics, metabolism, pathology)
• MicroRNAs (genetics, metabolism)
• Neoplasm Invasiveness (genetics)
• Skin Neoplasms (genetics, metabolism, pathology)
• T-Box Domain Proteins (genetics, metabolism)
• Up-Regulation