Characterization and Analysis of the Temporal and Spatial Dynamic of Several Enteritis Modeling Methodologies.

Abstract

Inflammatory bowel disease (IBD), such as Crohn's disease and ulcerative colitis, is a complex disease involving genetic, immune, and microbiological factors. A variety of animal models of IBD have been developed to study the pathogenesis of human IBD, but there is no model that can fully represent the complexity of IBD. In this study, we established two acute enteritis models by oral 3% DSS or intraperitoneal injection of anti-CD3 antibody, and two chronic enteritis models by feeding 3 cycles of 1.5% DSS or 3 months of the high-fat diet, respectively, and then examined the clinical parameters, histological changes, and cytokine expression profiles after the successful establishment of the models. Our results indicated that in 3% DSS-induced acute enteritis, the colorectal injury was significantly higher than that of the small intestine, while in anti-CD3 antibody-induced acute enteritis, the small intestine injury was significantly higher than that of colorectal damage. Besides, in the 1.5% DSS-induced chronic enteritis, the damage was mainly concentrated in the colorectal, while the damage caused by long-term HFD-induced chronic enteritis was more focused on the small intestine. Therefore, our work provides a reference for selecting appropriate models when conducting research on factors related to the pathogenesis of IBD or evaluating the potential diagnosis and treatment possibilities of pharmaceuticals.

• Authors: Huangru Xu, Fangfang Cai, Ping Li, Xiaoyang Wang, Yingying Yao, Xiaoyao Chang, Zhiqian Bi, Huisong Sun, Hongqin Zhuang, Zi-Chun Hua
• Journal: Frontiers in immunology (Front Immunol) Vol. 12 Pg. 727664 ( 2021) ISSN: 1664-3224 [Electronic] Switzerland
• PMID: 35003056 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021 Xu, Cai, Li, Wang, Yao, Chang, Bi, Sun, Zhuang and Hua.

Chemical References

• Antibodies
• CD3 Complex
• Cytokines
• Dextran Sulfate

Topics

• Acute Disease
• Administration, Oral
• Animals
• Antibodies (administration & dosage, adverse effects, immunology)
• CD3 Complex (immunology)
• Chronic Disease
• Cytokines (metabolism)
• Dextran Sulfate (administration & dosage, adverse effects)
• Diet, High-Fat (adverse effects)
• Disease Models, Animal
• Enteritis (chemically induced, immunology, metabolism, pathology)
• Feces (microbiology)
• Injections, Intraperitoneal
• Intestine, Large (injuries, metabolism)
• Intestine, Small (injuries, metabolism)
• Male
• Mice
• Mice, Inbred C57BL
• Microbiota (genetics)