Smart delivery of poly-peptide composite for effective cancer therapy.

Abstract

In the past decade, multifunctional peptides have attracted increasing attention in the biomedical field. Peptides possess many impressive advantages, such as high penetration ability, low cost, and etc. However, the short half-life and instability of peptides limit their application. In this study, a poly-peptide drug loading system (called HKMA composite) was designed based on the different functionalities of four peptides. The peptide compositions of HKMA composite from N-terminal to C-terminal were HCBP1, KLA, matrix metalloproteinase-2 (MMP-2)-cleavable peptide and albumin-binding domain. The targeting and lethality of HKMA to NSCLC cell line H460 sphere cells and the half-life of the system were measuredin vivo. The results showed that the HKMA composite had a long half-life and specific killing effect on H460 sphere cellsin vitroandin vivo. Our result proposed smart peptide drug loading system and provided a potential methodology for effective cancer treatment.

• Authors: Xin Yuan, Yingzhou Qin, Qingmei Tian, Cuijuan Liu, Xiangzhou Meng, Bo Qie, Fan Gao, Ying Huang, Guanghui Xu, Guang Yang, Yimin Zhu
• Journal: Biomedical materials (Bristol, England) (Biomed Mater) Vol. 17 Issue 2 (01 24 2022) ISSN: 1748-605X [Electronic] England
• PMID: 34996052 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2022 IOP Publishing Ltd.

Chemical References

• Antineoplastic Agents
• Peptide Fragments
• Recombinant Fusion Proteins

Topics

• Animals
• Antineoplastic Agents (chemistry, pharmacokinetics, pharmacology)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Drug Delivery Systems (methods)
• Female
• Humans
• Mice, Inbred BALB C
• Peptide Fragments (chemistry, genetics)
• Protein Domains (genetics)
• Recombinant Fusion Proteins (chemistry, genetics, pharmacokinetics, pharmacology)