Scopoletin inhibits PDGF-BB-induced proliferation and migration of airway smooth muscle cells by regulating NF-κB signaling pathway.

Abstract

BACKGROUND:

Asthma is a common chronic inflammatory disease of the airway, and airway remodeling and the proliferation mechanism of airway smooth muscle cells (ASMCs) is of great significance to combat this disease.

OBJECTIVE:

To assess possible effects of scopoletin on asthma and the potential signaling pathway.

MATERIALS AND METHODS:

ASMCs were treated PDGF-BB and scopoletin and subjected to cell viability detection by CCK-8 assay. Cell migration of ASMCs was determined by a wound closure assay and transwell assay. The protein level of MMP2, MMP9, calponin and α-SMA were measured using western blot. The levels of NF-κB signaling pathway were detected by Western blotting.

RESULTS:

Scopoletin inhibited proliferation of PDGF-BB - induced ASMCs. Also it suppressed the migration and invasion of PDGF-BB - induced ASMCs. We further showed that Scopoletin regulated phenotypic transition of ASMCs. Mechanically, Scopoletin inhibited proliferation and invasion of ASMCs by regulating NF-κB signaling pathway.

CONCLUSIONS:

We therefore thought Scopoletin could serve as a promising drug for the treatment of asthma.

• Authors: Zhongxiang Fan, Dan Tang, Qiang Wu, Qun Huang, Jie Song, Qiping Long
• Journal: Allergologia et immunopathologia (Allergol Immunopathol (Madr)) Vol. 50 Issue 1 Pg. 92-98 ( 2022) ISSN: 1578-1267 [Electronic] Singapore
• PMID: 34965643 (Publication Type: Journal Article)

Chemical References

• NF-kappa B
• Becaplermin
• Scopoletin

Topics

• Airway Remodeling
• Asthma
• Becaplermin
• Cell Proliferation
• Cells, Cultured
• Humans
• Myocytes, Smooth Muscle
• NF-kappa B (metabolism)
• Scopoletin (pharmacology)
• Signal Transduction