Lithium augments GM-CSA generation in canine cyclic hematopoiesis.

Abstract

Cyclic hematopoiesis in gray collie dogs can be cured by lithium treatment. We examined the mechanism of lithium's effect by developing an assay for the canine equivalent of GM-CSF (called GM-CSA). Phytohemagglutinin (PHA)-stimulated canine blood mononuclear cells produce GM-CSA in a dose-dependent manner; this GM-CSA stimulates more neutrophil-containing colonies than does endotoxin-treated dog serum. Production of GM-CSA by PHA-stimulated normal dog cells was not altered by lithium. However, cells from gray collies during their neutrophilic period increased their GM-CSA when lithium (2 mEq/L) was added to low doses of PHA, whereas neutropenic gray collie cells did not. These data suggest that lithium could modulate cyclic hematopoiesis by increasing intramedullary GM-CSA at the time when marrow neutrophilic progenitor cells are at their nadir.

• Authors: W P Hammond, E R Rodger, D C Dale
• Journal: Blood (Blood) Vol. 69 Issue 1 Pg. 117-23 (Jan 1987) ISSN: 0006-4971 [Print] United States
• PMID: 3491636 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Cyclosporins
• Interleukin-3
• Phytohemagglutinins
• Lithium

Topics

• Animals
• Cells, Cultured
• Cyclosporins (pharmacology)
• Dog Diseases (drug therapy)
• Dogs
• Female
• Hematologic Diseases (drug therapy, veterinary)
• Hematopoiesis (drug effects)
• Interleukin-3 (biosynthesis)
• Lithium (pharmacology, therapeutic use)
• Macrophages (physiology)
• Male
• Neutrophils (physiology)
• Phytohemagglutinins (pharmacology)