Abstract | OBJECTIVE: METHODS: Septic mouse model was established by cecal ligation and puncture. Then circPTK2 expression was detected and the role of circPTK2 in myocardial damage was assessed after circPTK2 expression was silenced using Ad-sh-circHIPK3. The subcellular localization of circPTK2 was analyzed. Besides, the binding relation between circPTK2 and microRNA (miR)-29b-3p and between miR-29b-3p and BCL2 antagonist/killer 1 (BAK1) was verified. The expression of miR-29b-3p and BAK1 in the myocardium was detected. Functional rescue was conducted to evaluate the role of miR-29b-3p and BAK1 in cardiomyocyte apoptosis in septic mice. RESULTS: CircPTK2 was highly expressed in the myocardium of septic mice, while circPTK2 silencing relieved the cardiac function and reduced inflammatory reaction and cardiomyocyte apoptosis of septic mice. Mechanically, circPTK2 competitively bound to miR-29b-3p to upregulate BAK1 mRNA level. Inhibition of miR-29b-3p and BAK1 overexpression could counteract the protective role of circPTK2 silencing in the myocardium of septic mice. CONCLUSION: CircPTK2 is overexpressed in the myocardium of septic mice. CircPTK2 competitively bound to miR-29b-3p to upregulate BAK1 mRNA level, to promote cardiomyocyte apoptosis, inflammatory response, and myocardial damage of the myocardium of septic mice.
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Authors | Hui Xiao, Quanzhu Fu, Li Min |
Journal | International archives of allergy and immunology
(Int Arch Allergy Immunol)
Vol. 183
Issue 5
Pg. 552-565
( 2022)
ISSN: 1423-0097 [Electronic] Switzerland |
PMID | 34915498
(Publication Type: Journal Article)
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Copyright | © 2021 S. Karger AG, Basel. |
Chemical References |
- Bak1 protein, mouse
- MicroRNAs
- RNA, Circular
- RNA, Messenger
- bcl-2 Homologous Antagonist-Killer Protein
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Topics |
- Animals
- Apoptosis
(genetics)
- Mice
- MicroRNAs
(genetics, metabolism)
- Myocytes, Cardiac
(metabolism)
- RNA, Circular
(genetics)
- RNA, Messenger
(metabolism)
- Sepsis
(genetics, metabolism)
- bcl-2 Homologous Antagonist-Killer Protein
(genetics, metabolism)
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