A Cancer-Related microRNA Signature Shows Biomarker Utility in Multiple Myeloma.

Abstract

Multiple myeloma (MM) is the second most common hematological malignancy, arising from terminally differentiated B cells, namely plasma cells. miRNAs are small non-coding RNAs that participate in the post-transcriptional regulation of gene expression. In this study, we investigated the role of nine miRNAs in MM. CD138+ plasma cells were selected from bone marrow aspirates from MM and smoldering MM (sMM) patients. Total RNA was extracted and in vitro polyadenylated. Next, first-strand cDNA synthesis was performed using an oligo-dT-adapter primer. For the relative quantification of the investigated miRNAs, an in-house real-time quantitative PCR (qPCR) assay was developed. A functional in silico analysis of the miRNAs was also performed. miR-16-5p and miR-155-5p expression was significantly lower in the CD138+ plasma cells of MM patients than in those of sMM patients. Furthermore, lower levels of miR-15a-5p, miR-16-5p, and miR-222-3p were observed in the CD138+ plasma cells of MM patients with osteolytic bone lesions, compared to those without. miR-125b-5p was also overexpressed in the CD138+ plasma cells of MM patients with bone disease that presented with skeletal-related events (SREs). Furthermore, lower levels of miR-223-3p were associated with significantly worse overall survival in MM patients. In conclusion, we propose a miRNA signature with putative clinical utility in MM.

• Authors: Aristea-Maria Papanota, Paraskevi Karousi, Christos K Kontos, Pinelopi I Artemaki, Christine-Ivy Liacos, Maria-Alexandra Papadimitriou, Tina Bagratuni, Evangelos Eleutherakis-Papaiakovou, Panagiotis Malandrakis, Ioannis Ntanasis-Stathopoulos, Maria Gavriatopoulou, Efstathios Kastritis, Margaritis Avgeris, Meletios-Athanasios Dimopoulos, Andreas Scorilas, Evangelos Terpos
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 22 Issue 23 (Dec 05 2021) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 34884950 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• MIRN125 microRNA, human
• MIRN15 microRNA, human
• MIRN16 microRNA, human
• MIRN222 microRNA, human
• MIRN223 microRNA, human
• MicroRNAs
• SDC1 protein, human
• Syndecan-1

Topics

• Adult
• Aged
• Biomarkers, Tumor (genetics)
• Female
• Gene Expression Profiling (methods)
• Gene Expression Regulation, Neoplastic
• Humans
• Male
• MicroRNAs (genetics)
• Middle Aged
• Multiple Myeloma (genetics, immunology)
• Survival Analysis
• Syndecan-1 (metabolism)