Abstract | OBJECTIVE: This study aims to investigate the regulatory effect of hypoxia on human periodontal ligament stem cells (PDLSCs) through RNA sequencing ( RNA SEQ). Human PDLSCs were cultured in normoxia (20% O2 ) or hypoxia (2% O2 ). MATERIAL AND METHODS: RESULTS: In total, 15 miRNAs, 449 lncRNAs, and 53 circRNAs were differentially expressed. Among them, 21 circRNAs, 262 lncRNAs, 5 miRNAs, and 5 mRNAs were selected to construct competing endogenous RNA ( ceRNA) networks. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were carried out to explore potential related pathways and regulatory functions. Several ceRNA axes ( lncRNA-FTX/ circRNA-FAT1-hsa-miR-4781-3p-SMAD5 and circRNA LPAR1-hsa-miR-342-3p-ADAR) may provide a theoretical basis on the study of osteogenic differentiation of PDLSCs under hypoxia. CONCLUSION: This study revealed that the expression profiles of circRNAs, lncRNAs, and miRNAs had changed significantly in PDLSCs cultured in 2% O2 ; specific circRNAs/lncRNAs may play a competitive role in the differentiation of PDLSCs.
|
Authors | Yu Ye, Lin Fu, Liu Liu, Tong Xiao, Ana Gloria Cuba Manduca, Jinhua Yu |
Journal | Oral diseases
(Oral Dis)
Vol. 29
Issue 3
Pg. 1197-1213
(Apr 2023)
ISSN: 1601-0825 [Electronic] Denmark |
PMID | 34874587
(Publication Type: Journal Article)
|
Copyright | © 2021 Wiley Periodicals LLC. |
Chemical References |
- RNA, Circular
- RNA, Long Noncoding
- MicroRNAs
- RNA, Messenger
|
Topics |
- Humans
- RNA, Circular
(genetics, metabolism)
- RNA, Long Noncoding
(genetics)
- Periodontal Ligament
(metabolism)
- Osteogenesis
(genetics)
- MicroRNAs
(genetics, metabolism)
- RNA, Messenger
(metabolism)
- Stem Cells
(metabolism)
- Gene Regulatory Networks
|