Hydrogen sulphide protects mice against the mutual aggravation of cerebral ischaemia/reperfusion injury and colitis.

Abstract

This study was undertaken to determine whether ischaemia/reperfusion (I/R)-induced brain injury and dextran sulfate sodium (DSS)-induced colitis in mice are related. A cerebral I/R model of mice was established by blocking the bilateral common carotid arteries; 3% DSS in drinking water was administered to mice for 7 days to induce colitis; mice with cerebral I/R and colitis were administered DSS for 7 days from the third day onwards after acute cerebral I/R. Brain damage and intestinal inflammation were also tested. The results revealed that cerebral I/R induced brain damage and a marked increase in glial fibrillary acidic protein (GFAP) expression and upregulation of Rho-associated coiled coil-forming protein kinase (RhoA/ROCK) pathway in mouse hippocampal tissues. However, in the colon tissues of mice with colitis, we found a reduction in GFAP. In addition, the expression of endogenous hydrogen sulphide (H2S) synthase reduced in mice brain tissues with cerebral I/R injury, as well. as in mouse colon tissues with colitis. Interestingly, the cerebral I/R-induced pathological changes in mouse brain tissues were aggravated by colitis, colitis mediated colon inflammation, and pathological changes in intestinal tissues had deteriorated when the mice suffered cerebral I/R 2 days before DSS administration. However, brain injury and colon inflammation in mice suffering from both cerebral I/R and colitis were ameliorated by NaHS, an exogenous H2S donor. Furthermore, we found that NaHS promoted the transformation of astrocytes from "A1" to "A2" type. These findings reveal that cerebral I/R injury and colitis are related, the mechanism is correlated with endogenous H2S deficiency.

• Authors: Yanyu Ding, Bo Liu, Yang Zhang, Fang Fang, Xueyan Li, Song Wang, Jiyue Wen
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 914 Pg. 174682 (Jan 05 2022) ISSN: 1879-0712 [Electronic] Netherlands
• PMID: 34871558 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 Elsevier B.V. All rights reserved.

Chemical References

• Glial Fibrillary Acidic Protein
• Protective Agents
• Sulfides
• glial fibrillary astrocytic protein, mouse
• Dextran Sulfate
• rho-Associated Kinases
• sodium bisulfide

Topics

• Animals
• Brain Ischemia (drug therapy, immunology, metabolism)
• Brain-Gut Axis (drug effects, physiology)
• Colitis (drug therapy, immunology)
• Dextran Sulfate (pharmacology)
• Disease Models, Animal
• Glial Fibrillary Acidic Protein (metabolism)
• Mice
• Protective Agents (pharmacology)
• Reperfusion Injury (drug therapy, metabolism)
• Signal Transduction (drug effects)
• Sulfides (pharmacology)
• Treatment Outcome
• Up-Regulation
• rho-Associated Kinases (metabolism)