In the past few years, we have demonstrated the efficacy of a nanoparticle system, super
carbonate apatite (sCA), for the in vivo delivery of
siRNA/
miRNA.
Intravenous injection of sCA loaded with small RNAs results in safe, high
tumor delivery in mouse models. To further improve the efficiency of
tumor delivery and avoid liver toxicity, we successfully developed an inorganic nanoparticle device (iNaD) via high-frequency ultrasonic pulverization combined with PEG blending during the production of sCA. Compared to sCA loaded with 24 μg of
miRNA, systemic administration of iNaD loaded with 0.75 μg of
miRNA demonstrated similar delivery efficiency to mouse
tumors with little accumulation in the liver. In the mouse therapeutic model, iNaD loaded with 3 μg of the
tumor suppressor small
RNA MIRTX resulted in an improved anti-
tumor effect compared to sCA loaded with 24 μg. Our findings on the bio-distribution and
therapeutic effect of iNaD provide new perspectives for future nanomedicine engineering.