LncRNA FGD5-AS1 functions as an oncogene to upregulate GTPBP4 expression by sponging miR-873-5p in hepatocellular carcinoma.

Abstract

The long non-coding FGD5-AS1 (LncFGD5-AS1) has been reported to be a novel carcinogenic gene and participant in regulating tumor progression by sponging microRNAs (miRNAs). However, the pattern of expression and the biological role of FGD5-AS1 in hepatocellular carcinoma (HCC) remains largely unknown. The expression level of FGD5-AS1 in tumor tissues and cell lines was measured by RT-qPCR. CCK-8, EdU, flow cytometry, wound healing, and transwell chamber assays were performed to investigate the role of FGD5-AS1 in cell proliferation, apoptosis, migration, and invasion in HCC. Dual luciferase reporter, and RNA pull-down assays were performed to identify the regulatory interactions among FGD5-AS1, miR-873-5p and GTP-binding protein 4 (GTPBP4). We found that the expression of FGD5-AS1 was upregulated in HCC tissues and cell lines. Moreover, the knockdown of FGD5-AS1 suppressed cell proliferation, migration and invasion, and induced apoptosis in HCC cells. Further studies demonstrated that FGD5-AS1 could function as a competitive RNA by sponging miR-873-5p in HCC cells. Moreover, GTPBP4 was identified as direct downstream target of miR-873-5p in HCC cells and FGD5-AS1mediated the effects of GTPBP4 by competitively binding with miR-873-5p. Taken together, this study demonstrated the regulatory role of FGD5-AS1 in the progression of HCC and identified the miR-873-5p/GTPBP4 axis as the direct downstream pathway. It represents a promising novel therapeutic strategy for HCC patients.

• Authors: Nuobei Zhang, Hao Shen, Shenan Huang, Fenfen Wang, Huifang Liu, Fen Xie, Lei Jiang, Xin Chen
• Journal: European journal of histochemistry : EJH (Eur J Histochem) Vol. 65 Issue 4 (Nov 16 2021) ISSN: 2038-8306 [Electronic] Italy
• PMID: 34783233 (Publication Type: Journal Article)

Chemical References

• MIRN873 microRNA, human
• MicroRNAs
• Neoplasm Proteins
• Nuclear Proteins
• RNA, Long Noncoding
• RNA, Neoplasm
• GTP-Binding Proteins
• GTPBP4 protein, human

Topics

• Carcinoma, Hepatocellular (genetics, metabolism)
• GTP-Binding Proteins (biosynthesis, genetics)
• Gene Expression Regulation, Neoplastic
• Hep G2 Cells
• Humans
• Liver Neoplasms (genetics, metabolism)
• MicroRNAs (biosynthesis, genetics)
• Neoplasm Proteins (biosynthesis, genetics)
• Nuclear Proteins (biosynthesis, genetics)
• Oncogenes
• RNA, Long Noncoding (genetics, metabolism)
• RNA, Neoplasm (genetics, metabolism)
• Up-Regulation