Protective Effect of Conditioned Media of Human Fetal Dermal Mesenchymal Stem Cells Can Inhibit Burn-induced Microvascular Hyperpermeability.

Abstract

Burns often cause loss of skin barrier protection, fluid exudation, and local tissue edema, which hinder functional recovery. Effectively improving the quality of deep burn wound healing, shortening the wound healing time, and reducing tissue fluid leakage are urgent problems in the medical field. Human mesenchymal stem cells (MSCs) can effectively stabilize vascular endothelial injury. Fetal dermal MSCs (FDMSCs) are a newly discovered source of MSCs derived from the skin of accidentally aborted fetuses. However, the effect of FDMSCs on vascular permeability remains poorly understood. In this study, conditioned media from FDMSCs (F-CM) extracted from fetal skin tissue was prepared. The effect of F-CM on vascular permeability was evaluated using the internal circulation method FITC-dextran in vivo, and several in vitro assays, including cell viability assay, transwell permeability test, immunofluorescence, and western blotting. Altogether, our results demonstrate that F-CM could inhibit burn-induced microvascular hyperpermeability by increasing the protein expression levels of occludin and VE-cadherin, while restoring the expression of endothelial F-actin, and providing the foundation of a novel therapy for the treatment of burns with F-CM.

• Authors: Yi Pan, Xiao Wang, Xinglei Wang, Fei Shan, Maoying Wang, Jixun Zhang, Jingjuan Zhang, Shanshan Jia, Ya Jiao, Yongjun Qi, Hongmin Gong, Duyin Jiang
• Journal: Journal of burn care & research : official publication of the American Burn Association (J Burn Care Res) Vol. 43 Issue 3 Pg. 735-741 (05 17 2022) ISSN: 1559-0488 [Electronic] England
• PMID: 34665252 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © Crown copyright 2021.

Chemical References

• Culture Media, Conditioned

Topics

• Burns (metabolism, therapy)
• Capillary Permeability
• Culture Media, Conditioned (metabolism, pharmacology)
• Humans
• Mesenchymal Stem Cells
• Wound Healing