Inhibition of UBA5 Expression and Induction of Autophagy in Breast Cancer Cells by Usenamine A.

Abstract

Breast cancer is now the most common type of cancer worldwide, surpassing lung cancer. This issue is further worsened by the lack of effective therapies for the disease. Recent reports indicate that the inhibition of ubiquitin-like modifier-activating enzyme 5 (UBA5) can impede tumor development. However, there have been few reports regarding UBA5-inhibiting compounds. This work studied usenamine A, a natural product from the lichen Usnea longissimi that exhibits UBA5-inhibitory effects. Bioinformatics analysis was performed using public databases, and the anti-proliferative ability of usenamine A in breast cancer cells was examined through MTS and colony formation assays. Flow cytometry and western blot analysis were also conducted to examine and analyze cell cycle arrest and apoptosis. In addition, LC3B-RFP and UBA5 expression plasmids were used for the analysis of usenamine A-induced autophagy. According to the bioinformatics analysis results, UBA5 was upregulated in breast cancer. According to in vitro studies, usenamine A displayed prominent anti-proliferative activity and resulted in G2/M phase arrest in MDA-MB-231 cells. Moreover, usenamine A induced autophagy and endoplasmic reticulum stress in MDA-MB-231 cells. In conclusion, the findings support the potential of usenamine A as an agent that can attenuate the development and progression of breast cancer.

• Authors: Bo Fang, Zijun Li, Yinda Qiu, Namki Cho, Hee Min Yoo
• Journal: Biomolecules (Biomolecules) Vol. 11 Issue 9 (09 11 2021) ISSN: 2218-273X [Electronic] Switzerland
• PMID: 34572561 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Messenger
• UBA5 protein, human
• Ubiquitin-Activating Enzymes

Topics

• Apoptosis (drug effects, genetics)
• Autophagy (drug effects, genetics)
• Breast Neoplasms (genetics, pathology)
• Cell Cycle Checkpoints (drug effects, genetics)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Endoplasmic Reticulum Stress (drug effects, genetics)
• Female
• Gene Expression Regulation, Neoplastic (drug effects)
• Gene Ontology
• Humans
• Molecular Docking Simulation
• Neoplasm Invasiveness
• Prognosis
• Protein Interaction Maps (drug effects, genetics)
• RNA, Messenger (genetics, metabolism)
• Reproducibility of Results
• Survival Analysis
• Ubiquitin-Activating Enzymes (antagonists & inhibitors, metabolism)