Naringin promotes osteogenesis and ameliorates osteoporosis development by targeting JAK2/STAT3 signalling.

Abstract

Osteoporosis is a systemic bone metabolism disorder, which increases the risk of fractures, and in severe cases it may cause disability or even death. An important factor contributing to osteoporosis is the imbalance between bone formation and resorption. Naringin was reported to promote osteoblast differentiation, thus enhancing bone formation and alleviating osteoporosis development. However, the signalling pathways related to the regulatory mechanism of naringin in osteoporosis development are not clear. Proliferation of bone mesenchymal stem cells (BMSCs) treated with naringin in vitro was detected by CCK-8. An osteogenesis differentiation medium supplemented with naringin was applied to explore the effects of naringin on BMSC osteogenic differentiation, as detected by Alizarin red staining. Ovariectomy (OVX)-induced postmenopausal osteoporosis (PMOP) rats were orally administered with naringin. Dual-energy X-ray absorptiometry (DEXA) and micro-CT were applied to measure bone mineral density (BMD), bone volume/total volume (BV/TV), trabecula thickness (Tb.Th), trabecula number (Tb.N), trabecular separation (Tb.Sp) and bone surface/bone volume (BS/BV). H&E staining was performed to show pathological changes of the femur in PMOP rats after naringin treatment. Bone metabolism indicators were assessed by ELISA. We found that naringin suppressed the activation of the JAK2/STAT3 pathway. Naringin promoted BMSC proliferation and osteogenic differentiation. Furthermore, naringin alleviates bone loss and improves abnormal bone metabolism of PMOP rats. Collectively, naringin promotes BMSC osteogenic differentiation to ameliorate osteoporosis development by targeting JAK2/STAT3 signalling.

• Authors: Wang Wang, Jie Mao, Yan Chen, Jing Zuo, Lin Chen, Yajing Li, Yingqian Gao, Qibin Lu
• Journal: Clinical and experimental pharmacology & physiology (Clin Exp Pharmacol Physiol) Vol. 49 Issue 1 Pg. 113-121 (01 2022) ISSN: 1440-1681 [Electronic] Australia
• PMID: 34525226 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2021 John Wiley & Sons Australia, Ltd.

Chemical References

• Flavanones
• STAT3 Transcription Factor
• Stat3 protein, rat
• Jak2 protein, rat
• Janus Kinase 2
• naringin

Topics

• Animals
• Female
• Flavanones (therapeutic use)
• Janus Kinase 2 (metabolism)
• Mesenchymal Stem Cells (drug effects)
• Osteogenesis (drug effects)
• Osteoporosis (drug therapy)
• Ovariectomy
• Rats
• Rats, Inbred Lew
• Rats, Sprague-Dawley
• STAT3 Transcription Factor (metabolism)
• Signal Transduction (drug effects)