Abstract | BACKGROUND: METHODS: A systematic review and meta-analysis were performed using PRISMA guidelines (Research Registry ref. 5874). Data were extracted from PubMed/MEDLINE and ScienceDirect databases for studies published from January 1, 2000 to December 31, 2019. Studies reporting antimicrobial resistance and virulence factors of UPEC isolated in confirmed urinary tract infections (≥105CFU/ml) were eligible. Prevalence of antimicrobial resistance and virulence factors of UPEC were estimated using random-effects meta-analysis model. Estimates with 95% confidence intervals, I-square (I2) statistic, and Cochran's Q test were computed using the score statistic and the exact binomial method by incorporating the Freeman-Tukey double arcsine transformation of proportions. RESULTS: Our search returned 2504 hits, of which 13 studies were included in the meta-analysis, totalling 1888 UPEC isolates. Highest antimicrobial resistance rates were observed among the antibiotic class of tetracycline in 69.1% (498/721), followed by sulphonamides in 59.3% (1119/1888), quinolones in 49.4% (1956/3956), and beta-lactams in 36.9% (4410/11964). Among beta-lactams, high resistance was observed in aminopenicillins in 74.3% (1157/1557) and first generation cephalosporins in 38.8% (370/953). Meanwhile, virulence factors with highest prevalence were immune suppressors (54.1%) followed by adhesins (45.9%). Taken individually, the most observed virulence genes were shiA (92.1%), CSH (80.0%), fimH/MSHA (75.3%), traT (75.1%), sisA (72.2%), iucD (65.7%), iutA (61.8%), kpsMTII (60.6%), and PAI (55.2%). CONCLUSIONS: The increased antibiotic resistance of UPEC isolates was demonstrated and suggested a need for reassessment of empirical therapies in urinary tract infections treatment caused by this pathogen. In addition, this pathotype exhibited diverse surface and secreted virulence factors.
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Authors | Gabriel Kambale Bunduki, Eva Heinz, Vincent Samuel Phiri, Patrick Noah, Nicholas Feasey, Janelisa Musaya |
Journal | BMC infectious diseases
(BMC Infect Dis)
Vol. 21
Issue 1
Pg. 753
(Aug 04 2021)
ISSN: 1471-2334 [Electronic] England |
PMID | 34348646
(Publication Type: Journal Article, Meta-Analysis, Systematic Review)
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Copyright | © 2021. The Author(s). |
Chemical References |
- Anti-Bacterial Agents
- Virulence Factors
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Topics |
- Anti-Bacterial Agents
(pharmacology, therapeutic use)
- Drug Resistance, Bacterial
- Escherichia coli Infections
(drug therapy, epidemiology)
- Humans
- Urinary Tract Infections
(drug therapy, epidemiology)
- Uropathogenic Escherichia coli
(genetics)
- Virulence Factors
(genetics)
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