Abstract |
Circular RNAs ( circRNAs) are a class of novel non-coding RNAs that are vital in modulating gene expression and biological processes. Nevertheless, in cervical cancer (CC), the role of circRNA is much less investigated. In this work, circ_0000228 expression in CC is measured and circ_0000228's function and related mechanism are investigated. Quantitative real-time quantitative polymerase chain reaction (qRT-PCR) was utilized to examine the expression levels of circ_0000228, microRNA-195-5p (miR-195-5p) and lysyl oxidase-like protein 2 (LOXL2). Western blotting was employed to examine LOXL2 protein expression in CC cell lines. CC cell lines with circ_0000228 knockdown were constructed, and the CCK-8 experiment and Transwell experiment were executed to investigate the effect of circ_0000228 on the malignant characteristics of CC cells. Furthermore, a dual- luciferase reporter gene experiment was applied to validate the targeting relationship between circ_0000228 and miR-195-5p, miR-195-5p and LOXL2. In this study, we demonstrated that circ_0000228 showed a remarkable up-modulation in CC tissues and cell lines. Circ_0000228 knockdown repressed the growth and metastatic potential of CC cells. Mechanistically, circ_0000228 facilitated CC progression through sponging miR-195-5p and up-modulating LOXL2 expression. We conclude that circ_0000228 is an oncogenic circRNA, which participates in promoting CC progression via regulating the miR-195-5p/LOXL2 axis.
|
Authors | Shimei Liu, Bingqing Li, Ying Li, Huaihua Song |
Journal | Bioengineered
(Bioengineered)
Vol. 12
Issue 1
Pg. 4397-4406
(12 2021)
ISSN: 2165-5987 [Electronic] United States |
PMID | 34308761
(Publication Type: Journal Article)
|
Chemical References |
- MIRN195 microRNA, human
- MicroRNAs
- RNA, Circular
- Amino Acid Oxidoreductases
- LOXL2 protein, human
|
Topics |
- Amino Acid Oxidoreductases
(genetics, metabolism)
- Cell Line, Tumor
- Cervix Uteri
(pathology)
- Female
- Gene Knockdown Techniques
- Humans
- MicroRNAs
(genetics, metabolism)
- RNA, Circular
(genetics, metabolism)
- Uterine Cervical Neoplasms
(genetics, metabolism, pathology)
|