CXC chemokines and their receptors in black rockfish (Sebastes schlegelii): Characterization, evolution analyses, and expression pattern after Aeromonas salmonicida infection.

Abstract

Chemokines are crucial regulators of cell mobilization for development, homeostasis, and immunity. Chemokines signal through binding to chemokine receptors, a superfamily of seven-transmembrane domain G-coupled receptors. In the present study, seventeen CXC chemokine ligands (SsCXCLs) and nine CXC chemokine receptors (SsCXCRs) were systematically identified from Sebastes schlegelii genome. Phylogeny, synteny, and evolutionary analyses were performed to annotate these genes, indicating that the tandem duplications (CXCL8, CXCL11, CXCL32, CXCR2, and CXCR3), the whole genome duplications (CXCL8, CXCL12, CXCL18, and CXCR4), and the teleost-specific members (CXCL18, CXCL19, and CXCL32) led to the expansion of SsCXCLs and SsCXCRs. In addition, SsCXCLs and SsCXCRs were ubiquitously expressed in nine examined healthy tissues, with high expression levels observed in head kidney, liver, gill and spleen. Moreover, most SsCXCLs and SsCXCRs were significantly differentially expressed in head kidney, liver, and gill after Aeromonas salmonicida infection, and exhibited tissue-specific and time-dependent manner. Finally, protein-protein interaction network (PPI) analysis indicated that SsCXCLs and SsCXCRs interacted with a few immune-related genes such as interleukins, cathepsins, CD genes, and TLRs, etc. These results should be valuable for comparative immunological studies and provide insights for further functional characterization of chemokines and receptors in teleost.

• Authors: Yuqing Li, Pei Zhang, Chengbin Gao, Min Cao, Ning Yang, Xingchun Li, Chao Li, Qiang Fu
• Journal: International journal of biological macromolecules (Int J Biol Macromol) Vol. 186 Pg. 109-124 (Sep 01 2021) ISSN: 1879-0003 [Electronic] Netherlands
• PMID: 34242645 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 Elsevier B.V. All rights reserved.

Chemical References

• Chemokines, CXC
• Fish Proteins
• Receptors, Chemokine

Topics

• Aeromonas salmonicida (immunology, pathogenicity)
• Animals
• Chemokines, CXC (genetics)
• Databases, Genetic
• Fish Proteins (genetics)
• Furunculosis (genetics, immunology, microbiology)
• Gene Expression Regulation
• Gene Regulatory Networks
• Gram-Negative Bacterial Infections (genetics, immunology, microbiology, veterinary)
• Host-Pathogen Interactions
• Immunity, Innate (genetics)
• Perciformes (genetics, immunology, microbiology)
• Phylogeny
• Protein Interaction Maps
• Receptors, Chemokine (genetics)
• Signal Transduction
• Time Factors