CCT5 interacts with cyclin D1 promoting lung adenocarcinoma cell migration and invasion.

Abstract

Cyclin D1 (CCND1) has been identified as a metastatic promoter in various tumors including lung adenocarcinoma (LUAD), a subtype of non small cell lung cancer (NSCLC). The previous observation revealed that CCND1 was upregulated in NSCLC and predicted poor prognosis of LUAD patients. In this study, we examined a chaperonin containing TCP1 subunit 5 (CCT5) protein interacts with CCND1 in LUAD. Immunofluorescence demonstrated the co-localization of CCT5 and CCND1 protein in LUAD cells. CCT5 expression was detected with both immunohistochemistry (IHC) and bioinformatics analyses. Similar with the expression pattern of CCND1, CCT5 displayed a high level in LUAD tissues compared to non cancerous lung specimens. Patients with high CCT5 expression showed a significant shorter overall survival relative to those with low expression level. Furthermore, upregulated CCT5 exhibited significant positive correlation with TNM stage of LUAD patients in both IHC analyses and bioinformatics. Knocking down CCT5 remarkably inhibited LUAD cell migration and invasion in vitro by inactivating PI3K/AKT and its downstream EMT signals, which could abrogated the accelerated migration and invasion caused by CCND1 overexpression. In summary, our study discovered a highly expressed protein CCT5 in LUAD which interacted with CCND1 and promoted migration and invasion of LUAD cells by positively moderating PI3K/AKT-induced EMT pathway.

• Authors: Yiliang Meng, Liu Yang, Xiao Wei, Hongcheng Luo, Yingying Hu, Xingyu Tao, Jingjing He, Xuesong Zheng, Qunying Xu, Kunxiang Luo, Guifang Yu, Qisheng Luo
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 567 Pg. 222-229 (08 27 2021) ISSN: 1090-2104 [Electronic] United States
• PMID: 34217974 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

Chemical References

• CCT5 protein, human
• Cyclin D1
• Chaperonin Containing TCP-1

Topics

• Adenocarcinoma of Lung (metabolism, pathology)
• Cell Line, Tumor
• Cell Movement
• Chaperonin Containing TCP-1 (metabolism)
• Cyclin D1 (metabolism)
• Humans
• Lung Neoplasms (metabolism, pathology)
• Neoplasm Invasiveness (pathology)
• Protein Interaction Maps