In the current study, the underlying anti-metastatic mechanism of
melatonin contained in some edible plants was explored in association with transmembrane
protease serine 4 (TMPRSS4) mediated
metastasis and epithelial-mesenchymal transition (EMT) signaling in human HCT15 and SW620
colorectal cancer cells. Here, TMPRSS4 was highly expressed in HCT15, but was weakly expressed in SW620 cells.
Melatonin exerted weak cytotoxicity, decreased invasion, adhesion, and migration, and attenuated the expression of TMPRSS4,
cyclin E,
pro-urokinase-type
plasminogen activator (pro-uPA), p-
signal transducer and activator of transcription 3 (p-STAT3), p-
focal adhesion kinase (p-FAK), Snail and increased the expression of
E-cadherin, p27, pp38 and p-Jun N-terminal
kinases (p-JNK) in HCT15 cells. Conversely, overexpression of TMPRSS4 reduced the ability of
melatonin to activate
E-cadherin and reduce Snail. Furthermore, even in SW620 cells transfected with TMPRSS4-overexpression plasmid,
melatonin effectively suppressed invasion and migration along with decreased expression of Snail,
cyclin A,
cyclin E, pro-uPA and p-FAK and increased expression of
E-cadherin and p27. Overall, these findings provide evidence that
melatonin suppresses
metastasis in
colon cancer cells via inhibition of TMPRSS4 mediated EMT.