Associations Between Betatrophin with Irisin and Metabolic Factors: Effects of Two Exercise Trainings in Diabetic Rats.

Abstract

BACKGROUND:

Irisin and betatrophin are involved in insulin resistance. We investigated the effects of aerobic and resistance exercise (AE/RE) and de-training (cessation of the training after the AE/RE) on betatrophin, irisin and some metabolic factors in rats.

METHODS:

Wistar rats were assigned into six groups: non-diabetic rats (C), non-diabetic rats that performed AE/RE, diabetic rats (Dia), and diabetic rats that performed AE/RE (Dia+AE and Dia+RE). Diabetes was induced by high-fat diet/streptozotocin model. The rats de-trained for four weeks after the 12-week exercise training. Blood samples were analyzed by enzyme-linked immunosorbent assay (ELISA) method and statistical analyses were performed using repeated measures and 1-way analysis of variance (ANOVA).

RESULTS:

The 12-week ET improved homeostasis model assessment of IR (HOMA-IR), high-density lipoprotein cholesterol (HDL-C), total cholesterol (TC) and triglycerides (TG) in the trained diabetic groups (p<0.05). ET reduced betatrophin level in the Dia+RE but not in the Dia+AE group. Positive correlations between betatrophin and body weight (r=0.547; p<0.01), and HOMA-IR (r=0.461; p<0.05) but a negative correlation with LDL-C and TC (r=-0.684, r=-0.669; both p<0.01) were observed, whereas no significant correlation was found between betatrophin and HDL-C and TG (r=-0.225, r=-0.360; both p>0.05). Betatrophin was correlated with irisin in the healthy rats but not the diabetic rats (p<0.01).

CONCLUSIONS:

It seems that RE has greater efficiency than AE in reducing betatrophin level and irisin resistance. However, de-training caused most of the improvements resulting from RE to be lost, but not the improvements resulting from AE.

• Authors: Hassan Tavassoli, Ali Heidarianpour
• Journal: The American journal of the medical sciences (Am J Med Sci) Vol. 362 Issue 5 Pg. 496-505 (11 2021) ISSN: 1538-2990 [Electronic] United States
• PMID: 34077706 (Publication Type: Journal Article)
• Copyright: Copyright © 2021. Published by Elsevier Inc.

Chemical References

• Angiopoietin-Like Protein 8
• Cholesterol, HDL
• FNDC5 protein, rat
• Fibronectins
• Insulin
• Triglycerides

Topics

• Angiopoietin-Like Protein 8 (blood)
• Animals
• Cholesterol, HDL
• Diabetes Mellitus, Experimental
• Fibronectins (blood)
• Insulin
• Insulin Resistance
• Physical Conditioning, Animal (methods)
• Rats
• Rats, Wistar
• Triglycerides